Cloning of full-length cDNA of gene encoding dihydrodipicolinate synthase from Carthamus tinctorius and construction of plant expression vector / 中草药
Chinese Traditional and Herbal Drugs
; (24): 1629-1634, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-852852
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To clone the full-length cDNA sequence of DHDPS gene encoding the key enzyme DHDPS in lysing biosynthesis pathway of Carthamus tinctorius, and to construct the plant expression vector.Methods:
The dihydrodipicolinate synthase (DHDPS) gene fragment was acquired according to the sequence of transcriptome in C. tinctorius, and the full-length cDNA sequence of CtDHDPS gene from C. tinctorius seeds was cloned by RT-PCR and RACE technologies. The pBasta-DHDPS plant expression vector was constructed using traditional molecular cloning and recombination technique.Results:
Bioinformatics analysis showed that the full-length cDNA of CtDHDPS was 1309 bp, open reading frame was 954 bp, encoding a polypeptide of 317 amino acids, the theoretical isoelectric point of the coded protein was 5.93, and the molecular weight was about 34 750.79. The plant expression vector pBasta-DHDPS was successfully constructed by traditional molecular cloning and recombinant technique.Conclusion:
The full-length sequence of CtDHDPS gene is obtained and the plant expression vector is successfully constructed, which lays a foundation for the further study on the mechanism of CtDHDPS in regulation of essential amino acid metabolism.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2017
Tipo de documento:
Artigo