Simultaneous determination of 23 plant growth regulator residues in Chinese materia medica by ultra performance liquid chromatography-tandem mass spectrometry / 中草药

ABSTRACT

Objective: To establish an ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) method for the simultaneous determination of 23 plant growth regulator (PGR) residues in Chinese materia medica (CMM). Methods: Samples were extracted with acetontrile, and then determined by UPLC-MS/MS directly without further clean up. The matrix-matched external standard method was used for quantitative analysis. Results: The calibration curves showed good linearity in each range with correlation coefficients greater than 0.99. The limits of detection (LODs) were 0.01-20.80 ng/mL for the 23 PGRs spiked in Codonopsis pilosula and Angelica dahurica. The recoveries of the 23 PGRs spiked in C. pilosula and A. dahurica at three levels of 0.01, 0.04, and 0.1 mg/kg were in the range of 71.0%-101.4%, the relative standard deviation (RSDs) were 0.8%-15.2%. Commonly used CMM including eight species (63 batches) was analyzed by this method. Conclusion: The method proved to be simple, rapid, sensitive, accurate, and suitable for the simultaneous determination of 23 PGR residues in CMM