Screening and validation of reference genes for quantitative RT-PCR analysis in Ampelopsis grossedentata / 中草药
Chinese Traditional and Herbal Drugs
; (24): 1192-1198, 2017.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-852918
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To screen reference genes for real time quantitative PCR (qRT-PCR) research in Ampelopsis grossedentata.Methods:
On the basis of the conserved sequences among plant species, six candidate reference genes (including Actin, 18 S-rRNA, GAPDH, α-Tubulin, β-Tubulin, and UBQ) were cloned from A. grossedentata by RT-PCR in this study. The expression stability of each reference gene in different tissues (shoot tip, young leaf, mature leaf, old leaf, stem, and root) were analyzed by three softwares (GeNorm, NormFinder, and BestKeeper), followed by validation of the expression pattern of AgPAL by qRT-PCR.Results:
Actin, 18 S-rRNA, and GAPDH expressed most stably in all samples and were suitable for reference genes, which were further confirmed by the transcript level analysis result of AgPAL in different tissues.Conclusion:
This is the first report on the screening and validation of reference genes for qRT-PCR in A. grossedentata, which benefits future studies on gene expression in this species.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2017
Tipo de documento:
Artigo