Identification of chemical constituents in Qiling Wenshen Xiaonang Formula by UPLC-ESI-IT-TOF/MS / 中草药
Chinese Traditional and Herbal Drugs
; (24): 1094-1100, 2016.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-853605
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To establish a method for the content identification of chemical constituents in Qiling Wenshen Xiaonang Formula by using UPLC-ESI-IT-TOF/MS, which can infer the potential active ingredients in the formula, combining with literature.Methods:
The chromatography separation was performed on a Waters ACQUITY UPLC BEH C18 column (100 mm × 2.1 mm, 1.7 μm) with acetonitrile-0.1% formic acid in water by gradient elution. Electrospray ionization (ESI) source was applied and operated in both positive and negative ion mode.Results:
A total of 36 chemical constituents were identified by ESI/MS through comparison on chemical standards and literature data, including 19 flavonoids, 11 phenolic acids, four triterpenoid saponins, one sesquiterpene glycoside, and one alkaloid.Conclusion:
This study introduces a comprehensive analysis method for chemical ingredients in Qiling Wenshen Xiaonang Formula, and lays a foundation for the further analysis on the substance basis research. The results provide a significant guidance for the substance basis research and quality control of compound Chinese materia medica.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Tipo de estudo:
Estudo diagnóstico
/
Guia de prática clínica
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2016
Tipo de documento:
Artigo