Screening and identification of available endophytes in roots of Panax ginseng / 中草药

ABSTRACT

Objective: To investigate the resource situation of endophytes in the roots of Panax ginseng (ginseng) of Jilin province and to select endophytes with antagonistic effect on pathogenic fungi of ginseng. Methods: Strains with antagonistic effect were screened by applying mixed strains method during preliminary screening and adopting antagonism method of fermentation liquor in re-screening. Besides, the selected endophytes were identified via 16S rDNA and ITS methods. Results: One hundred and thirty-three endophytes were isolated from ginseng. After preliminary screening and re-screening, four strains with good antagonistic effect on pathogenic fungi of ginseng were selected: B16, B25, B69, and F32. Among them, B16, B25, and B69 showed an inhibitory effect on six pathogenic fungi; F32 had a good inhibitory effect on Seclerotinia schinseng, Phytophthora cactorum, and Fusarium solani, with an inhibitory zone diameter of greater than 35 mm; According to the identification, B16, B25, B69, and F32 were Bacillus methylotrophicus, B. amyloliquefaciens, B. vallismortis, and Penicillium daleae, respectively. Conclusion: Endophytes in ginseng are diversified and there also exist strains with high antagonistic effect, which can be a good source for biocontrol bacterium and fungus of ginseng diseases.