Analysis on changing rules of four active components alignment in Glycyrrhizae Radix et Rhizoma Praeparata cum Melle and its compatibility carrier / 中草药

ABSTRACT

Objective: To study the changing rules of Glycyrrhizae Radix et Rhizoma Praeparata cum Melle (GRRPM) with the active components alignment (ACA, liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin) of tonifying spleen and replenishing qi. Methods: Taking single herbs, their medicine pairs, or their medicine compounds as test sample, the sample solution was prepared by traditional water decoction and determined by HPLC. Results: The contents of liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin in GRRPM were (12.14±0.31), (3.25±0.19), (38.75±1.77), and (1.40±0.25) mg/mL; The ACA contents in GRRPM-Ginseng Radix et Rhizoma (GRR) were (2.87±0.04), (0.74±0.02), (6.42±0.18), and (0.37±0.03) mg/mL; The ACA contents in GRRPM-Atractylodis Macrocephalae Rhizoma (AMR) were (5.99±0.01), (2.16±0.09), (22.40±1.80), and (0.77±0.02) mg/mL; The ACA contents in Sijunzi Decotion (SD) were (2.99±0.03), (0.81±0.04), (10.05±0.31), and (0.32±0.03) mg/mL. Conclusion: Four main active components in the efficacy of the test components have almost the same change rule: GRRPM≤GRRPM-AMR≤SD≤GRRPM-GRR; The percentage change intervals of the ACA (liquiritin-liquiritigenin-glycyrrhizin acid-isoliquiritinin) are (7.76-9.34):(2.00-2.81):(17.35-31.41):1. The total contents in single herbs are three times as much as in compatibility components, so the Chinese materia medica (CMM) are often used in the type of formula, which indicates that the utilized space of ACA resources is larger, ACA has the obvious effect of correspondence, and the quality standard of CMM should be marked ACA and medicinal components of biological potency as indicators.