Effects of liposome nanoparticles of hydrazinocurcumin on cell proliferation, apoptosis, invasion, and migration in MDA-MB-231 cells / 中草药

ABSTRACT

Objective: To investigate the effects of liposome nanoparticles of hydrazinocurcumin (HC-NPs) on cells proliferation, apoptosis, invasion, and migration in human breast cancer MDA-MB-231 cells. Methods: Liposome HC-NPs were prepared by film-sonication method and then were used to treat human breast cancer MDA-MB-231 cells. The treated cell survival was analyzed by MTT; Morphological changes were detected by Liu staining; The cell cycle and apoptosis were investigated by FCM assay; Then invasion and migration of the cells were analyzed by Transwell. Moreover, the expression of proteins correlated to cell cycle arrest, apoptosis, invasion, and migration were detected by Western blotting. Results: The cell proliferation was inhibited by HC-NPs, the cells became round in shape, and the cells were arrested in G 2/M phase, the ratio of apoptosis was increased (P<0.01), the cells invasion and migration were restrained. The expression of p-STAT3, CyclinD1, Survivin, Bcl-2, and MMP-9 were down-regulated, while the expression of Bax was up-regulated. Conclusion: The effects of HC-NPs on the suppression of cell proliferation, invasion, and migration, and induction of cells apoptosis may be related to the inhibition of STAT3 activation.