Inhibitory mechanism of oridonin on G2/M phase arrest in SGC-7901 cell growth / 中草药
Chinese Traditional and Herbal Drugs
; (24): 2024-2026, 2010.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-855731
Biblioteca responsável:
WPRO
ABSTRACT
Objective:
To investigate the effect of oridonin on G2/M phase arrest of human stomach cancer SGC-7901 cell growth in vitro and its molecular mechanism.Methods:
The inhibition of oridonin on SGC-7901 cells was detected by MTT assay. Effect of oridonin on the phase distribution of SGC-7901 cell cycle was observed by flow cytometry (FCM). Western blotting was used to analyze the expression of Cdk1 and CyclinB1.Results:
IC50 of oridonin on SGC-7901 cells was 15. 64 μmol/L. The effect of oridonin on SGC-7901 cells presented the increasing percentages of cells in G2/M phase as the concentration of oridonin increased. With the increase of oridonin dose, the expression of Cdk1 and CyclinB1 proteins was remarkably decreased.Conclusion:
Oridonin can down-regulate the expression of Cdk1 and CyclinB1, which may be the mechanism of arresting SGC-7901 cells in G2/M phase.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Traditional and Herbal Drugs
Ano de publicação:
2010
Tipo de documento:
Artigo