Overcoming Cancer Multidrug Resistance by Doxorubicin-TiO2 Nanoparticles / 中国药学杂志
Chinese Pharmaceutical Journal
; (24): 1198-1202, 2018.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-858271
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE To investigate the effect of TiO2 nanoparticles on overcoming cancer multidrug resistance(MDR). METHODS:
Doxorubicin-TiO2 nanoparticles(DTN) were prepared, the K562/DOX cells were chosen as the model cells. And doxorubicin solution(F-DOX) and doxorubicin liposomes(DOX-L) were also prepared as the control. The MTT assay were measured, and the amount of doxorubicin in the K562/DOX cells at different time were determined by HPLC. The P-gp expression were detected by flow cytometry.RESULTS:
The MTT assay shows that IC50 of group DTN were lower than that of group F-DOX. The uptake test shows that amount of doxorubicin in K562/DOX cells of group DTN was 1.23 times of group DOX-L when in 4 h, and the efflux test shows that amount of doxorubicin in K562/DOX cells was 1.18 times of group DOX-L. The flow cytometry result revealed that the effect of TiO2 nanoparticles on overcoming MDR maybe through down-regulating the expression of P-gp in K562/DOX cells.CONCLUSION:
The TiO2 nanoparticles are a new inorganic materials-based nanoparticles which promising approach to overcome MDR.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Idioma:
Chinês
Revista:
Chinese Pharmaceutical Journal
Ano de publicação:
2018
Tipo de documento:
Artigo