Simultaneous determination of seven nucleosides in inonotus obliquus by HPCE / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a high performance capillary electrophoresis (HPCE) method for simultaneous determination of seven nucleosides and nucleobases, including adenine, uracil, adenosine, hypoxanthine, uridine, guanosine, and inosine in Inonotus obliquus samples of different batches. METHODS: Based on the mode of HPCE, uncoated fused silica capillary (75 μm × 64.5 cm, 56 cm of effective length) was used with separation voltage of 20 kV. The 55 mmol·L-1 sodium borate solution was selected as the running buffer solution (pH 9.5). The detection wavelength was set at 260 nm. The sample was injected at 5 kPa × 6 s and column temperature was maintained at 30℃. RESULTS: Good linear relationship was obtained between the response peak areas and the concentrations of all the seven nucleosides (r > 0.998 8). The average recoveries of the method were between 96.46%-102.12%. The contents of the seven nucleosides in the Inonotus obliquus samples of different origins were different. CONCLUSION: The established method is reliable, accurate, and can be used for the quality control of Inonotus obliquus.