Evaluation of four kinds of rapid identification technologies of microbials in pharmaceutical products / 中国药学杂志

ABSTRACT

OBJECTIVE: To establish a evaluation method of microbial identification technology by comparing four different microbial identification methods. METHODS: A total of 50 tests by API microbial identification system, VITEK 2 compact system, Riboprinter system, and MicroSeq ID DNA sequencing system were performed against 19 standard strains and 10 wild strains. The accuracy and reproducibility of these methods were evaluated. RESULTS: All the four identification technologies could be used to identify specified micro-organisms, but they had difference in other strains. Molecular biotechnology was better than biochemical identification in accuracy and reproducibility. CONCLUSION: The users should establish suitable acceptance criteria for accuracy and reproducibility, taking into account method capability.