In vivo tissue distribution of hydroxyapatite nanoparticles modified with aminopropyltriethoxysilane / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the in vivo tissue distribution of hydroxyapatite nanoparticles (HANPs) and provide important information for the in vivo biosafety evaluation of HANPs. METHODS: The HANPs were modified with aminopropyltriethoxysilane (APTS) to introduce amino groups on the surface. The modified HANPs were labeled with 125I and injected into mice. A γ-counter was used to quantitatively assess the radioactivity of the tissues. The accumulation of HANPs in the tissues was expressed as the percentage injected dose per gram tissue (%ID · g-1). RESULTS: The HANPs mainly accumulated in the lung, liver, and spleen. One day post-injection, the accumulation in these tissues was over 5% ID · g-1. During 30 d after the injection, the accumulation of HANPs in the lung decreased quickly, while the accumulation in the liver and spleen reduced moderately and maintained at more than 2% ID · g-1. CONCLUSION: This study indicates that lung, liver and spleen are the main tissues for the in vivo biosafety evaluation of HANPs. KEY