Human recombinant neutrophil inhibitory factor and hirulog hybrid (TNHH) protects against focal cerebral ischemia injury in rats / 中国药学杂志

ABSTRACT

OBJECTIVE: To investigate the protective effects of human recombinant neutrophil inhibitory factor and hirulog hybrid (TNHH) on focal cerebral ischemia in rats. METHODS: Middle cerebral artery occlusion model was prepared in rats to imitate the focal cerebral ischemia. Rats were divided into five groups: (1)blank, (2)sham, (3)focal cerebral ischemia, (4)ischemia + vehicle and (5)ischemia + TNHH(6.75 mg · kg-1). After persistent ischemia for 12 h, neurologic deficit score was assayed, and then scarified the animals to measure the brain infraction area by TTC staining, the brain pathological damage by HE staining, and the levels of inflammatory factor TNF-α and IL-1β in serum respectively by using ELISA and radio-immunity assay. RESULTS: TNHH could significantly improve the neurological outcome, decrease the infraction area, alleviate brain edema and pathological injury and reduce the levels of TNF-α and IL-1β in rats suffered persistent focal cerebral ischemia for 12 h. CONCLUSION: TNHH has significant anti-inflammatory and anti-edema effects, which could effectively alleviate focal cerebral ischemia injury in rats.