Hairy cell transformation of human peripheral blood lymphocytes by Coxiella burnetii
Yonsei Medical Journal
; : 11-21, 1993.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-87576
Biblioteca responsável:
WPRO
ABSTRACT
Hairy cell (HC) transformation of human peripheral blood lymphocytes (PBL) by Coxiella burnetii was studied to clarify the significance of persistency of C. burnetii in a hairy cell line (designated "TOL"). TOL cells which exhibited HC characteristics in hairy cell leukemia (HCL) were persistently infected with C. burnetii. Two strains of C. burnetii, our isolate from TOL cells and the original isolate in 1935, the Nine Mile strain from American Type Culture Collection (ATCC, U.S.A), were inoculated to PBL cultures. HC transformation not only by our isolates (87%) but also by Nine Mile strain (100%) was demonstrated in an average of 20 days. The original observation that Coxiella induced HC transformation in vitro was also confirmed in experiments with PBL exposed to C. burnetii in vivo. Spontaneous development of HC were observed in cultures of PBL only from coxiellemic cases (12/24) but not from C. burnetii negative cases (0/57). All HC cell lines (34) as determined by their morphology and cytochemical markers of HC in HCL remained infected with C. burnetii invariably.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Células Sanguíneas
/
Linfócitos
/
Microscopia Eletrônica de Varredura
/
Leucemia de Células Pilosas
/
Linhagem Celular
/
Transformação Celular Neoplásica
/
Coxiella burnetii
Limite:
Humanos
Idioma:
Inglês
Revista:
Yonsei Medical Journal
Ano de publicação:
1993
Tipo de documento:
Artigo