MicroRNA-135a in ABCA1-labeled Exosome is a Serum Biomarker Candidate for Alzheimer's Disease / 生物医学与环境科学(英文)
Biomedical and Environmental Sciences
; (12): 19-28, 2021.
Artigo
em Inglês
| WPRIM (Pacífico Ocidental)
| ID: wpr-878317
Biblioteca responsável:
WPRO
ABSTRACT
Objective@#In the present study, the ABCA1 was used as a label to capture specific exosomes, the level of ABCA1-labeled exosomal microRNA-135a (miR-135a) was evaluated for the diagnosis of Alzheimer's disease (AD), especially in patients with early stages of AD.@*Methods@#This is a preliminary research focused on the levels of ABCA1 in WBCs, RBCs, HT-22 cells, and neuron cells. The diagnostic value of ABCA1-labeled exosomal miR-135a was examined using the CSF and serum of APP/PS1 double transgenic mice, and 152 patients with SCD, 131 patients with MCI, 198 patients with DAT, and 30 control subjects.@*Results@#The level of ABCA1 exosomes harvested from HT-22 cells and neuron culture medium was significantly higher compared to that of RBCs and WBCs ( @*Conclusion@#This study outlines a method to capture specific exosomes and detect them using immunological methods, which is more efficient for early diagnosis of AD.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Camundongos Transgênicos
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Biomarcadores
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Linhagem Celular
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MicroRNAs
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Eritrócitos
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Exossomos
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Doença de Alzheimer
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Disfunção Cognitiva
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Transportador 1 de Cassete de Ligação de ATP
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Leucócitos
Tipo de estudo:
Estudo de rastreamento
Limite:
Idoso, 80 anos ou mais
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Animais
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Feminino
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Humanos
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Masculino
Idioma:
Inglês
Revista:
Biomedical and Environmental Sciences
Ano de publicação:
2021
Tipo de documento:
Artigo