Genome sequencing of Streptomyces aureofaciens DM-1 and analysis of 6-demethylchlortetracycline biosynthesis gene cluster / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2685-2694, 2020.
Article
em Zh
| WPRIM
| ID: wpr-878521
Biblioteca responsável:
WPRO
ABSTRACT
Streptomyces aureofaciens DM-1 is a high-yielding 6-demethylchlortetracycline producer. The genome sequencing of DM-1 reveals a linear chromosome containing 6 824 334 bps nucleotides with GC content of 72.6%. In this genome, a total of 6 431 open reading frames were predicted by using glimmer 3.02, Genemark and Z-Curve softwares. Twenty-eight secondary metabolite biosynthetic gene clusters were uncovered by using AntiSMASH gene prediction software, including the complete 6-demethylchlortetracycline biosynthetic gene cluster. A frame-shift mutation in methyltransferase coding region was detected, which may result in the demethylation of chlortetracycline. The complete genome sequence of S. aureofaciens DM-1 provides basic information for functional genomics studies and selection of high-yielding strains for 6-demethylchlortetracycline.
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Texto completo:
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Base de dados:
WPRIM
Assunto principal:
Streptomyces aureofaciens
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Sequência de Bases
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Clortetraciclina
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Família Multigênica
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Demeclociclina
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2020
Tipo de documento:
Article