Rapid screening of single guide RNA targeting pig genome and the harvesting of monoclonal cells by microarray seal / 生物医学工程学杂志
Journal of Biomedical Engineering
; (6): 111-121, 2021.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-879256
Biblioteca responsável:
WPRO
ABSTRACT
The emergence of regular short repetitive palindromic sequence clusters (CRISPR) and CRISPR- associated proteins 9 (Cas9) gene editing technology has greatly promoted the wide application of genetically modified pigs. Efficient single guide RNA (sgRNA) is the key to the success of gene editing using CRISPR/Cas9 technology. For large animals with a long reproductive cycle, such as pigs, it is necessary to screen out efficient sgRNA
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Suínos
/
Repetições Palindrômicas Curtas Agrupadas e Regularmente Espaçadas
/
Sistemas CRISPR-Cas
/
Edição de Genes
Tipo de estudo:
Estudo diagnóstico
/
Estudo de rastreamento
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Biomedical Engineering
Ano de publicação:
2021
Tipo de documento:
Artigo