Guiqi Dingnian Prescription Delays Senescence of Mouse Mesangial Cells by Regulating JAK2/STAT Pathway Activity / 中国实验方剂学杂志
Chinese Journal of Experimental Traditional Medical Formulae
; (24): 67-73, 2021.
Article
em Zh
| WPRIM
| ID: wpr-906394
Biblioteca responsável:
WPRO
ABSTRACT
Objective:To investigate the effects of Guiqi Dingnian prescription (GDP) on the expression of related molecules in Janus tyrosine kinase 2 (JAK2)/signal transducer and activator of transcription (JAK2/STAT) signaling pathway of <italic>D</italic>-galactose (<italic>D</italic>-gal)-induced senescent mesangial cells. Method:The senescent mouse mesangial cells induced by 10 g·L<sup>-1</sup> <italic>D</italic>-gal were continuously treated with 40 mg·L<sup>-1 </sup>GDP for three days. The senescence of the treated cells was determined by senescence-associated (SA)-<italic>β</italic>-gal staining. The cell cycle was detected by flow cytometry. The cell viability was analyzed using the cell counting kit-8 (CCK-8). The mRNA expression levels of tumor necrosis factor-<italic>α</italic> (TNF-<italic>α</italic>), interleukin-6 (IL-6), nuclear transcription factor-<italic>κ</italic>B (NF-<italic>κ</italic>B), and IL-1<italic>α</italic> were detected by real-time polymerase chain reaction (Real-time PCR). The protein expression levels of STAT1, phosphorylated STAT1 (p-STAT1), STAT3, and p-STAT3 in the JAK2/STAT signaling pathway were determined by Western blot. Result:CCK-8 results showed that the optimal concentration of GDP was 40 mg·L<sup>-1</sup>. Compared with the blank group, the positive rate of SA-<italic>β</italic>-gal in the model group was significantly higher(<italic>P</italic><0.01), the percentage of cells in G<sub>0</sub>/G<sub>1</sub> phase was significantly increased(<italic>P</italic><0.05), the percentage of cells in G<sub>2</sub>/M and S phase was significantly decreased(<italic>P</italic><0.01). The mRNA expression levels of TNF-<italic>α</italic>,IL-6,NF-<italic>κ</italic>B and IL-1<italic>α </italic>were significantly increased(<italic>P</italic><0.01). Compared with the model group, the model + GDP group exhibited significantly decreased SA-<italic>β</italic>-gal-positive cells (<italic>P</italic><0.05), reduced cells in the G<sub>0</sub>/G<sub>1</sub> phase (<italic>P</italic><0.05), increased cells in the G<sub>2</sub>/M and S phases (<italic>P</italic><0.01), and down-regulated TNF-<italic>α</italic>, IL-6, NF-<italic>κ</italic>B, and IL-1<italic>α </italic>mRNA expression (<italic>P</italic><0.05) and STAT1, p-STAT1, STAT3, and p-STAT3 protein expression (<italic>P</italic><0.05). Conclusion:GDP delays the senescence of mouse mesangial cells possibly by down-regulating the expression of related molecules in the JAK2/STAT pathway.
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Base de dados:
WPRIM
Tipo de estudo:
Prognostic_studies
Idioma:
Zh
Revista:
Chinese Journal of Experimental Traditional Medical Formulae
Ano de publicação:
2021
Tipo de documento:
Article