Application of loop-mediated isothermal amplification based point-of-care testing in laboratory medicine / 中华检验医学杂志

ABSTRACT

With the advancement of medical science, point-of-care testing (POCT), which is simple to operate, quick to respond, and does not rely on equipment and professional and technical personnel, is expected to realize continuous monitoring, diagnosis, management and screening of patients. It is an important development direction of the in vitro diagnostic industry. Combining the principles of new technology, POCT is inevitably developing towards the transformation from qualitative to precise quantitative under the premise of improving sensitivity and specificity. During the development of the POCT platform, the simple, fast, low-cost loop-mediated isothermal amplification (LAMP) technology with high-efficiency amplification characteristics plays an increasingly important role. This article summarizes the mechanism of the LAMP technology, the research progress, and the clinical application of the POCT platform based on the LAMP. The current deficiencies and future development directions of the POCT platform based on the LAMP are also discussed.