The Establishment and Identification of Acute Myeloid Leukemia NOD-SCID-IL2rg / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 1429-1435, 2021.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-922276
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To establish the in vivo traceable acute myeloid leukemia mice model with Luciferase-Expressing KG1a Cells.@*METHODS@#KG1a cells with stable luciferase gene expression (called as KG1a-Luc cells) were constructed by lentivirus transfection, then sifted out by puromycin. Eighteen male NOD-SCID-IL2rg@*RESULTS@#KG1a cells expressing luciferase stably were successfully obtained. The tumor luminescence wildly spread at day 17 captured by in vivo imaging. The KG1a-Luc tumor cells could be detected in the peripheral blood of the mice, with the average percentage of (16.27±6.66)%. The morphology and pathology result showed that KG1a-Luc cells infiltrate was detected in bone marrow, spleens and livers. The survival time of the KG1a-Luc mice was notably shorter as compared with those in the control group, the median survival time was 30.5 days (95%CI 0.008-0.260).@*CONCLUSION@#The acute myeloid leukemia NOD-SCID-IL2rg
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Leucemia Mieloide Aguda
/
Camundongos SCID
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Camundongos Endogâmicos NOD
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Modelos Animais de Doenças
/
Subunidade gama Comum de Receptores de Interleucina
/
Luciferases
Tipo de estudo:
Estudo diagnóstico
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2021
Tipo de documento:
Artigo