Changes of Serum IgM, IgG in Pig's Xenograft Perfusion and Immunofluorescence Changes of the Deposition of IgM, IgG in the Xenograft in Dogs / 대한흉부외과학회지
The Korean Journal of Thoracic and Cardiovascular Surgery
; : 467-472, 2007.
Artigo
em Coreano
| WPRIM (Pacífico Ocidental)
| ID: wpr-95015
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND:
Lung transplantation is the definitive therapy for end stage lung disorders. The success of allogenic lung transplantation has led to an increasing shortage of donor lungs from humans, including cadavers, and attention has now turned to transplantation of lungs from other species. However, there are many biological hurdles when using organs from other species because of hyperacute rejection after discordant xenotransplantation. MATERIAL ANDMETHOD:
Pigs (n=6, weighing 20~30 kg each) for the donors and mongrel dogs (n=6, weighing 20~28 kg each) for the recipients were used in this experiment. The left kidney of a pig was perfused to a mongrel dog for 30 minutes through the femoral artery and vein of the dog, and the right kidney was perfused for 30 minutes sequentially. Then, both lungs of the pig were perfused to the dog through the pulmonary artery and left atrium with using the same time intervals. The levels of IgM and IgG were measured from the blood and specimens of the kidney and lung.RESULT:
The average levels of serum IgM gradually decreased after the perfusion, but the average levels of serum IgG did not change from before to after perfusion. The immunohistochemical findings revealed decreased deposition of IgG and IgM after the perfusion.CONCLUSION:
We conclude that the levels of the serum natural antibodies would be decreased with pre-transplantation xenograft perfusion in the recipient and the occurrence rate of hyperacute rejection after transplantation would be decreased.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Perfusão
/
Artéria Pulmonar
/
Suínos
/
Doadores de Tecidos
/
Transplante Heterólogo
/
Veias
/
Cadáver
/
Imunoglobulina G
/
Imunoglobulina M
/
Imunofluorescência
Limite:
Animais
/
Humanos
Idioma:
Coreano
Revista:
The Korean Journal of Thoracic and Cardiovascular Surgery
Ano de publicação:
2007
Tipo de documento:
Artigo