A modified rapid microprocedure of determining unsaturated fatty acids from blood serum

ABSTRACT

1. This method for determining the 2- and 4-double bond fatty acid content in the blood serum is based on ultraviolet absorption spectroscopy of the isolated fatty acid2. The method is limited only to the determination of polyunsaturated fatty acids, due to the small amount of lipid fatty acids represented in the small amount of blood sample used3. The validity of the procedure has been tested by addition of certain amount of standards under assay to the unknown serum and the mixture analyzed to see how much of the material added is recovered4. Freeze-drying method had been introduced in the procedure to save time in connection with the evaporation of extract to dryness and prevent oxidation of the fatty acids. (Summary)