Expression of Hepatitis B Virus X Protein in Hepatocytes Suppresses CD8+ T Cell Activity
Immune Network
; : 126-134, 2010.
Article
em En
| WPRIM
| ID: wpr-96923
Biblioteca responsável:
WPRO
ABSTRACT
BACKGROUND: CD8+ T cells contribute to the clearance of Hepatitis B virus (HBV) infection and an insufficient CD8+ T cell response may be one of the major factors leading to chronic HBV infection. Since the HBx antigen of HBV can up-regulate cellular expression of several immunomodulatory molecules, we hypothesized that HBx expression in hepatocytes might affect CD8+ T cell activity. METHODS: We analyzed the activation and apoptosis of CD8+ T cells co-cultured with primary hepatocytes rendered capable of expressing HBx by recombinant baculovirus infection. RESULTS: Expression of HBx in hepatocytes induced low production of interferon-gamma and apoptosis of CD8+ T cells, with no effect on CD8 T cell proliferation. However, transcriptional levels of H-2K, ICAM-1 and PD-1 ligand did not correlate with HBx expression in hepatocytes. CONCLUSION: Our results suggest that HBx may inhibit CD8+ T cell response by regulation of interferon-gamma production and apoptosis.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Proteínas Virais
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Linfócitos T
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Hepadnaviridae
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Transativadores
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Vírus da Hepatite B
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Baculoviridae
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Interferon gama
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Apoptose
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Molécula 1 de Adesão Intercelular
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Hepatócitos
Idioma:
En
Revista:
Immune Network
Ano de publicação:
2010
Tipo de documento:
Article