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Bis (2-butoxyethyl) Phthalate Delays Puberty Onset by Increasing Oxidative Stress and Apoptosis in Leydig Cells in Rats / 生物医学与环境科学(英文)
Biomed. environ. sci ; Biomed. environ. sci;(12): 60-75, 2023.
Article em En | WPRIM | ID: wpr-970291
Biblioteca responsável: WPRO
ABSTRACT
OBJECTIVE@#This study investigated the effects of bis (2-butoxyethyl) phthalate (BBOP) on the onset of male puberty by affecting Leydig cell development in rats.@*METHODS@#Thirty 35-day-old male Sprague-Dawley rats were randomly allocated to five groups mg/kg bw per day that were gavaged for 21 days with BBOP at 0, 10, 100, 250, or 500 mg/kg bw per day. The hormone profiles; Leydig cell morphological metrics; mRNA and protein levels; oxidative stress; and AKT, mTOR, ERK1/2, and GSK3β pathways were assessed.@*RESULTS@#BBOP at 250 and/or 500 mg/kg bw per day decreased serum testosterone, luteinizing hormone, and follicle-stimulating hormone levels mg/kg bw per day (P < 0.05). BBOP at 500 mg/kg bw per day decreased Leydig cell number mg/kg bw per day and downregulated Cyp11a1, Insl3, Hsd11b1, and Dhh in the testes, and Lhb and Fshb mRNAs in the pituitary gland (P < 0.05). The malondialdehyde content in the testis significantly increased, while Sod1 and Sod2 mRNAs were markedly down-regulated, by BBOP treatment at 250-500 mg/kg bw per day (P < 0.05). Furthermore, BBOP at 500 mg/kg bw per day decreased AKT1/AKT2, mTOR, and ERK1/2 phosphorylation, and GSK3β and SIRT1 levels mg/kg bw per day (P < 0.05). Finally, BBOP at 100 or 500 μmol/L induced ROS and apoptosis in Leydig cells after 24 h of treatment in vitro (P < 0.05).@*CONCLUSION@#BBOP delays puberty onset by increasing oxidative stress and apoptosis in Leydig cells in rats.@*UNLABELLED@#The graphical abstract is available on the website www.besjournal.com.
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Texto completo: 1 Base de dados: WPRIM Assunto principal: Maturidade Sexual / Testículo / Testosterona / Ratos Sprague-Dawley / Apoptose / Estresse Oxidativo / Serina-Treonina Quinases TOR / Glicogênio Sintase Quinase 3 beta / Células Intersticiais do Testículo Limite: Animals Idioma: En Revista: Biomed. environ. sci Ano de publicação: 2023 Tipo de documento: Article
Texto completo: 1 Base de dados: WPRIM Assunto principal: Maturidade Sexual / Testículo / Testosterona / Ratos Sprague-Dawley / Apoptose / Estresse Oxidativo / Serina-Treonina Quinases TOR / Glicogênio Sintase Quinase 3 beta / Células Intersticiais do Testículo Limite: Animals Idioma: En Revista: Biomed. environ. sci Ano de publicação: 2023 Tipo de documento: Article