Preparation and immunogenicity evaluation of mRNA vaccine against porcine epidemic diarrhea / 生物工程学报
Chinese Journal of Biotechnology
; (12): 2624-2633, 2023.
Article
em Zh
| WPRIM
| ID: wpr-981220
Biblioteca responsável:
WPRO
ABSTRACT
Porcine epidemic diarrhea (PED) is a highly contagious disease that causes high mortality in suckling piglets. Although several licensed inactivated and live attenuated vaccines were widely used, the infection rate remains high due to unsatisfactory protective efficacy. In this study, mRNA vaccine candidates against PED were prepared, and their immunogenicity was evaluated in mice and pregnant sows. The mRNA PED vaccine based on heterodimer of viral receptor binding region (RBD) showed good immunogenicity. It elicited robust humoral and cellular immune responses in mice, and the neutralizing antibody titer reached 1:300 after a single vaccination. Furthermore, it induced neutralizing antibody level similar to that of the inactivated vaccine in pregnant sows. This study developed a new design of PED vaccine based on the mRNA-RBD strategy and demonstrated the potential for clinical application.
Palavras-chave
Texto completo:
1
Base de dados:
WPRIM
Assunto principal:
Suínos
/
Doenças dos Suínos
/
Vacinas Atenuadas
/
Vacinas Virais
/
Diarreia
/
Anticorpos Neutralizantes
/
Anticorpos Antivirais
Limite:
Animals
/
Pregnancy
Idioma:
Zh
Revista:
Chinese Journal of Biotechnology
Ano de publicação:
2023
Tipo de documento:
Article