RHD Gene Analysis of A Blood Donor with Del Phenotype / 中国实验血液学杂志
Journal of Experimental Hematology
; (6): 843-849, 2023.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-982139
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To analyze the RHD genotype of a blood donor with Del phenotype in Yunnan.@*METHODS@#Rh serological phenotype was identified. RHD gene was detected by PCR-SSP typing, and its 10 exons were sequenced. Exon 9 was amplified for sequencing and analysis. RHD zygosity was detected.@*RESULTS@#The Rh phenotype of this specimen was CcDelee. Genomic DNA exhibited a 1 003 bp deletion spanning from intron 8, across exon 9 into intron 9. The deletion breakpoints occurred between two 7-bp short tandem repeat sequences. There was no variation in the sequences of the remaining exons. The Rh hybridization box test showed that there was one RHD negative allele.@*CONCLUSION@#This specimen is Del type caused by deletion of RHD exon 9.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fenótipo
/
Sistema do Grupo Sanguíneo Rh-Hr
/
Doadores de Sangue
/
China
/
Éxons
/
Alelos
/
Genótipo
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Journal of Experimental Hematology
Ano de publicação:
2023
Tipo de documento:
Artigo