Haplotype and heteroplasmy in the HV II region of mtDNA in human blood detected by PCR-DGGE / 法医学杂志
Journal of Forensic Medicine
; (6): 30-32, 2007.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-983258
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To establish a simple and effective technique for detecting haplotype and heteroplasmy of mtDNA, and investigate their frequencies in Chinese Han population.@*METHODS@#The fragments from 29-290 nt of mtDNA HV II from peripheral leukocytes of 200 unrelated Wuhan Han individuals were analyzed by using PCR-DGGE technique.@*RESULTS@#Seventeen haplotypes were found in the range of 29-290 nt, and the haplotype diversity (HD) was 0.8844. The heterogeneity was observed from 4 individuals, and its frequency was 2%.@*CONCLUSION@#PCR-DGGE is a simple, sensitive and effective technique in analyzing polymorphism and heteroplasmy of mtDNA, and can be used in forensic practice.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Haplótipos
/
DNA Mitocondrial
/
China
/
Reação em Cadeia da Polimerase
/
Análise de Sequência de DNA
/
Heterogeneidade Genética
/
Povo Asiático
/
Eletroforese em Gel de Poliacrilamida
/
Genética Populacional
/
Mutação
Limite:
Humanos
País/Região como assunto:
Ásia
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2007
Tipo de documento:
Artigo