The number of circulating fibrocytes of skeletal muscle in rats after contusion / 法医学杂志
Journal of Forensic Medicine
; (6): 1-11, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-983702
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTION@#To investigate the time-dependent appearance of circulating fibrocytes of skeletal muscle in rats after contusion.@*METHODS@#The model of skeletal muscle wound was established in rat. The circulating fibrocytes in contused skeletal muscle were detected by CD45 and procollagen I double immunofluorescence staining method.@*RESULTS@#In the control group, CD45- and procollagen I-positive cells were not detected in skeletal muscle. A few CD45 cells were observed aged from 6 h to 1 d after contusion. A few CD45- and procollagen I-positive cells (fibrocytes) initially gathered in injury area 3d after injury. The ratio of positive fibrocytes significantly increased 5 d after injury. The ratio of fibrocytes was highest at 7 d after contusion and then decreased. The volume of fibrocytes showed bigger with injury time increase compared with 3 d group. The expression of procollagen I and CD45 were weakened at 14d after injury.@*CONCLUSION@#The circulating fibrocytes are detected in contused skeletal muscle in time-dependent pattern. Circulating fibrocytes may be a marker in the wound age determination for contused skeletal muscle.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Fatores de Tempo
/
Cicatrização
/
Imuno-Histoquímica
/
Biomarcadores
/
Distribuição Aleatória
/
Ratos Sprague-Dawley
/
Antígenos Comuns de Leucócito
/
Músculo Esquelético
/
Contusões
/
Colágeno Tipo I
Limite:
Animais
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo