Degenerate oligonucleotide primed-PCR technology establishment and its sensitivity test analysis / 法医学杂志
Journal of Forensic Medicine
; (6): 41-43, 2012.
Artigo
em Chinês
| WPRIM (Pacífico Ocidental)
| ID: wpr-983711
Biblioteca responsável:
WPRO
ABSTRACT
OBJECTIVE@#To establish a whole genome amplification testing system based on degenerate oligonucleotide primed-PCR (DOP-PCR) and to explore its reliability and sensitivity.@*METHODS@#DOP-PCR amplified production was detected by fluorescent labeled multiplex STR amplification and capillary electrophoresis detection system to determine reliability and sensitivity of DOP-PCR system.@*RESULTS@#DOP-PCR system was successfully established and the detection sensitivity reached 5 cells (30 pg) by pretreatment of DOP-PCR and then detection of STR genotyping.@*CONCLUSION@#The system established in this study is reliable and more testing sensitive for forensic trace evidence.
Texto completo:
Disponível
Base de dados:
WPRIM (Pacífico Ocidental)
Assunto principal:
Oligonucleotídeos
/
DNA
/
Amplificação de Genes
/
Reação em Cadeia da Polimerase
/
Reprodutibilidade dos Testes
/
Impressões Digitais de DNA
/
Sensibilidade e Especificidade
/
Primers do DNA
/
Eletroforese Capilar
/
Sequências de Repetição em Tandem
Limite:
Humanos
Idioma:
Chinês
Revista:
Journal of Forensic Medicine
Ano de publicação:
2012
Tipo de documento:
Artigo