The association between sperm quality and asymptomatic chlamydial infection in infertile men at a private fertility clinic in Nigeria

BACKGROUND: Association between chlamydia trachomatis infection and male infertility is debated in literature. There is little or no information from Nigeria. The study aimed to determine the prevalence of chlamydial infection and its association with sperm quality parameters among a symptomatic men that present for infertility treatment in a Nigeria facility. METHODS: A cross-sectional study conducted at a private assisted conception clinic in Lagos, Nigeria among 138 men seeking infertility care. Seminal fluid analysis and IgG Chlamydial serology were performed for each participant. Data obtained were analysed using SPSS; p was significant at <0.05. RESULTS: Of the 138 men screened, 13.9% were Chlamydia-positive. Twenty-one per cent of clients who tested positive to Chlamydia had predominantly immotile sperm, compared with 10.2% without the infection; 26.3% with non-progressive motility had Chlamydia, compared with 2.8% men who were not infected. These differences were statistically significant (p = 0.001). More of those (57.9%) with Chlamydia (compared to 33.1% without) had significant leukocyte counts (p = 0.037). There were no statistically significant differences in sperm count and percent motility between serologically positive and negative men. CONCLUSION: Positive Chlamydia serology is associated with non-progressive motility and leukocytospermia in infertile Nigerian men

Investigation of the antioxidant activity and quantification of the amount of heavy Metals and some vitamins in carrot

Background: Carrot is a root vegetable from the Umbelliferae family. It is a biennial plant grown for their edible root. Carrots are a good source of carbohydrates and minerals like Calcium, Phosphorus, Iron and Magnesium and may contain toxic amounts of metals as a result of run off effects. It is also rich in carotene, niacin, riboflavin, thiamine and vitamin C.Objectives: To determine the amounts of toxic heavy metals, quantify the amounts of vitamins A and E and investigate the antioxidant activities of Carrot.Method: The research investigated the antioxidant properties of carrot on the basis of the radical scavenging activity on DPPH (1,1-diphenyl-2-picryl hydrazyl), heavy metal analysis were carried out using Flame Atomic Absorption Spectrophotometer while analysis of vitamins was done using HPLC (High Performance Liquid Chromatography).Result: The carrot sample analyzed contained considerable amount of some toxic metals of interest (Cr 0.024, 0.105ppm; Cu 15.76, 30.95ppm; Fe 66.94, 103.95ppm; Zn 16.57, 44.22ppm; Pb 0.018, 0.021ppm) in the leaves and root respectively. The samples also contain a very good amount of the vitamin A (12.863, 44.977ppm) and Vitamin E (0.087, 0.22ppm) in leaves and root respectively. It also showed some antioxidant activity and test positive for most phytochemicals.Conclusion: The actual concentrations of the respective heavy metals found in two parts of the D. carota samples were within the threshold limit but there was slight variation in the amount present in the root D. carota as compared to its leaf. The root had more concentrations of the metals and this could be due to the fact that the root is more exposed to these metals during plant uptake

Self-Perceived Seizure Precipitants among Patients with Epilepsy in Middle-Belt of Nigeria

Aim: Patient's perception of seizure precipitant is crucial in epilepsy management; but it is often overlooked by physicians. This may be due to neglect and underestimation of its importance. This study looked at frequency and nature of self-perceived seizure precipitants among patients with epilepsy. Materials and Methods: A close-ended questionnaire-based study. Patients with active epilepsy (=2 attacks/year) were recruited from the neurology clinic of the University of Ilorin Teaching Hospital (UITH); Ilorin. Result: A total of 89 patients participated in the study and of these 41 (46.1) were males. Their median age was 30 (21-52) years and median age at seizure onset was 22 (15-46) years. The median seizure duration was five (2-14) years. More patients (46.1) had less than secondary school education and 12 (13.5) were uneducated. Generalized epilepsy was the predominant (68.6) seizure type. A total of 33 (37.1 subjects had =4 attacks/year; 29 (32.6) had 5-12 attacks/year; and 27 (30.3) 12 attacks/year. A total of 16 (18) subjects did not mention any seizure precipitant; whereas 73 (82.2) reported at least one specific seizure precipitant; of these; 62 (85) patients reported =2 precipitants. Stress (41); inadequate sleep (27); and head trauma (26) were the three leading seizure precipitants mentioned. Subject's age; sex; level of seizure control; and place of abode did not influence reported seizure precipitants. However; the more educated (12 years education) patients significantly reported stress as seizure precipitant (P 0.05). Most (80) patients rightly indicated that antiepileptic drug was the best treatment for their seizure control. Conclusion: The result of this study showed that the leading perceived seizure precipitants among epilepsy patients attending the neurology clinic of UITH were stress; inadequate sleep; head trauma; and demonic attacks and spells

Morphological Variation of Third Ventricle Using Computerized Tomography among Different Gender and Age Groups: A 5-Year Retrospective Study in Usmanu Danfodiyo University Teaching Hospital; Sokoto; North - West Nigeria

Background: Many methods have been described for measurements of the third ventricle as a means of evaluating brain atrophy during the normal aging process and disease. Enlargement of the cerebral ventricles is one of the most frequently replicated neurobiological findings in schizophrenia. The aim of this morphological study was to examine the range in the normal size of the third ventricle of individuals living in Sokoto and to assess its association with gender and age. Materials and Methods: All available brain CT in the Radiology Department of the Usmanu Danfodiyo University Teaching Hospital (UDUTH) Sokoto; Nigeria; from 2007 to 2012 (a 5-year period) and reported as normal by the radiologist were recruited for the study. Films were viewed on the computer monitor. Measurements were made with Dragon V 3.1.1 Philips and Neusoft Medical System Company Limited software; the software provides a meter rule with which measurements were done. Results: A total of 252 CT scan images where used in the study. Of this number; 156 (61.9) were CT scan images of males and 96 (38.1) were CT scan images of females. The mean width was 8.38 mm and mean anteroposterior length was 12.16 mm. These differences were statistically significant; P = 0.0209 (0.05). Conclusion: Our findings provide a base line data for the measurement of the third ventricles using CT scans in our environment and this may be applied in various clinical conditions involving the third ventricle

Preferred Choice of Health Facilities for Healthcare among Adult Residents in Ilorin Metropolis; Kwara State; Nigeria

Purpose: The choice of health facilities for healthcare by an individual is largely determined by several factors. This study aims to determine predictors of preferred choice of health facility for care. Methods: In a descriptive cross-sectional study; pre-tested semi-structure questionnaire was administered to 366 adults selected through a multi-stage sampling technique in Ilorin metropolis. Data collected were analysed using Epi Info software version 3.4.1 and level of significance set at p 0.05. Results: The preferred health facility for medical care was private hospitals (35.2) followed by pharmaceutical store (27.9) and 17.0for general/teaching hospitals and only 12.3for primary health care (PHC). Quick service and availability of drugs were the major reasons for their preference which were said to be better in private hospitals. Sex; marital status; educational status; occupation and city area where the respondents dwell are all associated with the preferred choice of health facility for care. Conclusion: This study has shown that the private sector is preferred to the public ones with regards to receiving healthcare and that within the public sector; the higher levels of health facilities are preferred to the primary health care centres. Improving the image and performance of the public health facilities especially the PHC is very important for appropriate utilization of health services

Knowledge and Attitude of Civil Servants in Osun State; Southwestern Nigeria Towards the National Health Insurance

Introduction: In Nigeria; inequity and poor accessibility to quality health care has been a persistent problem. This study aimed to determine knowledge and attitude of civil servants in Osun state towards the National Health Insurance Scheme (NHIS). Methodology: This is a descriptive; cross sectional study of 380 civil servants in the employment of Osun state government; using multi stage sampling method. The research instruments was pre-coded; semi structured; self administered questionnaires. Results: About 60were aware of out of pocket as the most prevalent form of health care financing; while 40were aware of NHIS; television and billboards were their main sources of awareness; However; none had good knowledge of the components of NHIS; 26.7knew about its objectives; and 30knew about who ideally should benefit from the scheme. Personal spending still accounts for a high as 74.7of health care spending among respondents but respondents believed that this does not cover all their health needs. Only 0.3have so far benefited from NHIS while 199 (52.5) of respondents agreed to participate in the scheme. A significant association exists between willingness to participate in the NHIS scheme and awareness of methods of options of health care financing and awareness of NHIS (P0.05) Conclusion: Poor knowledge of the objectives and mechanism of operation of the NHIS scheme characterised the civil servants under study. The poor knowledge of the components and fair attitude towards joining the scheme observed in this study could be improved upon; if stakeholders in the scheme could carry out adequate awareness seminars targeted at the civil servants

Phytochemical Screening and Antioxidant Activities of some Selected Medicinal Plants Used for Malaria Therapy in Southwestern Nigeria

Purpose: Oxidative stress has been shown to play an important role in the development of anaemia in malaria. Indeed; increase in total antioxidant status has been shown to be important in recovery from malaria. The antioxidant activities of four medicinal plants traditionally used in the treatment of malaria in southwestern Nigeria were determi- ned. Methods: The ethanolic extracts of the leaves of Carica papaya Linn. [Caricaceae] ; stem bark of Magnifera indica Linn. [Anacardiaceae]; leaves of Psidium guajava Linn. [Myrtaceae] and the leaves of Vernonia amygdalina Del. [Compositae]; were used in the present study. The plant parts commonly used in the locality in malaria therapy were employed in this study. The plants were screened for the presence of phytochemicals and; their effect on 2;2-Diphenyl-1-picryl-hydrazyl radical (DPPH) was used to determine their free radical scavenging activity. Results: Phytochemical screening of the plants showed the presence of flavonoids; terpenoids; saponins; tannins and reducing sugars. M. indica did not contain cardiac glycosides and alkaloids while; P. guajava also showed the absence of alkaloids and anthraquinones. Anthraquinones was similarly absent from V. amygdalina. Concentrations of the plant extracts required for 50inhibition of DPPH radical scavenging effect (IC50) were recorded as 0.04 mg/ml; 0.313 mg/ml; 0.58 mg/ml; 2.30 mg/ml and 0.054 mg/ml for P. guajava; M. Indica; C. papaya; V. amygdalina and Vitamin C; respectively. Conclusion : All the plants showed potent inhibition of DPPH radical scavenging activity; P. guajava being the most potent. The free radical scavenging (antioxidant) activities of these plants probably contribute to the effectiveness of the above plants in malaria therapy

Phytochemical Screening and Free Radical Scavenging Activities of the Fruits and Leaves of Allanblackia floribunda Oliv (Guttiferae)

Purpose: To compare the phytochemical constituents in the leaves and fruits of Allanblackia floribunda and determine their free radical scavenging activity. Methods: The fruit and leaves of AF collected from the uncultivated farmlands of Okeigbo; Ondo State; Nigeria; were dried; milled and extracted with methanol. Phytochemical screening was carried out according to standard procedures. Free radical scavenging activity was determined by measuring the decrease in the visible absorbance of 2;2-diphenyl-1-picrylhydrazyl (DPPH) on addition of the plant extract. The mean inhibitory concentration (IC50); which is the concentration of extract needed to decrease the initial absorbance of DPPH by 50was determined graphically. Total phenolic; flavonoids and proanthocyanidin contents were determined by spectro-photometric methods. Results: Alkaloids; anthraquinones; tannins; saponins; steroids; terpenoids; flavonoids and cardiac glycosides were found to be present in both the fruits and leaves. Only AF fruit contained phlobatannins. IC50 values of 0.01; 0.02 and 0.1 mg/ml were recorded for Vitamin E; AF leaves and AF fruits respectively. Total phenolic; total flavonoid and proanthocyanidin contents were 65; 0.07 and 2.38 mg/g of powdered plant material for AF fruits; and 12; 51.35; 19.5 mg/g of powdered plant material for AF leaves as gallic acid; rutin and catechin equivalents respectively. Conclusion: AF leaves are five times more potent as a free radical scavenger compared to the fruits though the fruit was found to contain a higher phenolic content

Phytochemical Screening and Free Radical Scavenging Activities of the Fruits and Leaves of Allanblackia floribunda Oliv (Guttiferae)

Purpose: To compare the phytochemical constituents in the leaves and fruits of Allanblackia floribunda and determine their free radical scavenging activity. Methods: The fruit and leaves of AF collected from the uncultivated farmlands of Okeigbo; Ondo State; Nigeria; were dried; milled and extracted with methanol. Phytochemical screening was carried out according to standard procedures. Free radical scavenging activity was determined by measuring the decrease in the visible absorbance of 2;2-diphenyl-1 -picrylhydrazyl (DPPH) on addition of the plant extract. The mean inhibitory concentration (IC50); which is the concentration of extract needed to decrease the initial absorbance of DPPH by 50was determined graphically. Total phenolic; flavonoids and proanthocyanidin contents were determined by spectro-photometric methods. Results: Alkaloids; anthraquinones; tannins; saponins; steroids; terpenoids; flavonoids and cardiac glycosides were found to be present in both the fruits and leaves. Only AF fruit contained phlobatannins. IC50 values of 0.01; 0.02 and 0.1 mg/ml were recorded for Vitamin E; AF leaves and AF fruits respectively. Total phenolic; total flavonoid and proanthocyanidin contents were 65; 0.07 and 2.38 mg/g of powdered plant material for AF fruits; and 12; 51.35; 19.5 mg/g of powdered plant material for AF leaves as gallic acid; rutin and catechin equivalents respectively. Conclusion: AF leaves are five times more potent as a free radical scavenger compared to the fruits though the fruit was found to contain a higher phenolic content

Hypertension among Nigerian children

A prospective study on hypertension in childhood among Nigerian children was carried out in five centres. Four hundred and four children had complete data and were admitted into this study; out of which 226 (55.9 per cent) were males and 173 (44.1 per cent) were females. Twenty-two of the children examined were hypertensive with blood pressure more than 120/80mmHg; which was maximal in the age range 10-12 years. Females were more affected than males. Thirty-six of the children have their systolic blood pressure above 140mmHg