ABSTRACT
Background: The growing desires to combat antibiotic resistance among pathogenic bacteria necessitate the need to search for new antimicrobials agents from other sources such as plants.Objectives: The present study investigated the antibacterial activities and bioactive components of Nymphaea lotus and Spondias mombin against selected clinical bacteria.Material and Methods: Extracts of N. lotus and S. mombin were prepared by 72 hours maceration in 70% methanol. The antimicrobial susceptibility testing (AST) of Escherichia coli, Klebsiella pneumoniae, Enterobacter aerogenes, Salmonella typhi, Staphylococcus aureus, Citrobacter freundi and, Klebsiella oxytoca against the two extracts was carried out by disk diffusion method while minimum inhibitory concentrations (MIC) and minimum bactericidal concentrations (MBC) was by agar-well dilution and broth dilution method, respectively. The bioactive compounds of the plants were identified by Gas Chromatography-Mass Spectrometry (GC-MS) analysis.Results: Extracts of N. lotus showed better antimicrobial activities than S. mombin against all the clinical bacterial isolates with an MIC range of 3.13 >12.5mg/mL compared to S. mombin with MIC range of 6.25 >12.5mg/mL. The GC-MS results revealed the presence of 21 and 25 compounds for N. lotus and S. mombin respectively. Benzoic acid derivatives were in abundance in both plants with approximately 71.5% and 82.1% in N. lotus and S. mombin respectively.Conclusions: The findings from this study provided further evidence on their ethno-botanical claims and additional information on the potentials of the studied plants as effective medicinal plants with antimicrobial activity against clinical bacteria. This highlights the need for continuous exploration of medicinal plants for novel compounds with better antimicrobial property as option for the treatment of resistant bacterial infection
Subject(s)
Anacardiaceae , Anti-Infective Agents , Nigeria , Nymphaea , Plant ExtractsABSTRACT
"Notre etude sur le traitement traditionnel de l'hypertension a porte sur la recette Nitrokoudang a base de Sclerocarya birrea et Vitex doniana. L'etude phytochimique par les reactions de caracterisation en tube et la chromatographie sur couche mince a permis de mettre en evidence des tanins; des flavonoides; des leucoanthocyanes; des oses et holosides. L'infuse a presente un grand nombre de substances anti-radical 1;1'diphenyl-2 picryhydrazyle (DPPH); ce qui presage un potentiel antioxydant de la recette; ceci est important dans la prise en charge de l'hypertension arterielle. Les extraits aqueux de la recette ont presente une activite diuretique dose dependante. L'infuse extemporane; prepare selon les indications du tradipraticien de sante; a presente le meilleur effet diuretique avec une excretion urinaire volumetrique de 170; le Furosemide a la dose de 20mg/kg; a presente 184. Chez les animaux traites par la recette; l'elimination du sodium a ete importante et le potassium a ete epargne. L'activite salidiuretique de l'infuse extemporanee a ete de 2 contre 2;62 pour le Furosemide et de 0;90 chez les souris traitees avec l'eau distillee. Ces proprietes de la recette ""Nitrokoudang"" justifient son utilisation dans la prise en charge de l'hypertension arterielle."