ABSTRACT
Randomly selected goat sera from north-western; central; and south-western regions of Uganda were analyzed parasitologically and serologically for evidence of anaplasmosis. Prevalence rates of 3.2pc by parasitemia; 4.8pc by card-agglutination test; and 12.9pc by DOT-ELISA combined with western blotting were established. Parasitologically positive samples were consistently serologically positive. Positive samples were all from either the north-western or south-western regions of the country. Goats in these regions graze with cattle and are presumable exposed to the same tick species. There was no evidence of clinical caprine anaplasmosis; whereas bovine anaplasmosis cases are very common. Rhipicephalus evertsi was frequently observed on goats which cograze with cattle
Subject(s)
Anaplasma/isolation & purification , Anaplasmosis/immunology , Antibodies , Goat Diseases/immunology , GoatsABSTRACT
The prevalence of bovine anaplasmosis was studied in 320 Zebu cattle randomly selected from three regions of Uganda: (Central; Southwestern and Northwestern) using dot-ELISA; Western immunoblotting; rapid card agglutination test (RCAT); capillary tube agglutination test (CAT); complement fixation test (CFT); and parasitological techniques. Dreid blood on Whatman filter paper No.1 was eluted in PBS 0.0Tween 20 prior to testing at an initial dilution of 1:25. The prevalences of parasitaemia were 25in the central region; 28in the southwestern region; and 35in the northwestern region; and the serological prevalence was lowest in the central and highest in the northwest. Overall; prevalence rates obtained by dot-ELISA (61.9) and Western immunoblotting (62.5) were 1.5 times those obtained by RCAT (41) and three times those obtained by CAT (22.5). The overall prevalence rates obtained by dot-ELISA and Western immunoblotting compared favourably with the CFT data. The present data utilizing dried blood on filter papers indicate that there is a high prevalence of anaplasmosis in those regions of Uganda surveyed; and confirm our observations and those of others that collecting blood on filter papers is a suitable technique for large scale screening and for seroepidemiological studies
Subject(s)
Agglutination Tests , Anaplasmosis , ParasitemiaABSTRACT
The prevalence of bovine anaplasmosis was studied in 320 Zebu cattle randomly selected from three regions of Uganda: (Central; Southwestern and Northwestern) using dot-ELISA; Western immunoblotting; rapid card agglutination test (RCAT); capillary tube agglutination test (CAT); complement fixation test (CFT); and parasitological techniques. Dried blood on Whatman filter paper No. 1 was eluted in PBS 0.05pc Tween 20 prior to testing at an initial dilution of 1:25. The prevalences of parasitaemia were 25pc in the central region; 28pc in the southwestern region; and 35pc in the northwestern region; and the serological prevalence was lowest in the central region and highest in the northwest. Overall; prevalence rates obtained by dot-ELISA (61.9pc) and Western immunoblotting (62.5pc) were 1.5 times those obtained by RCAT (41pc) and three times those obtained by CAT (22.5pc). The overall prevalence rates obtained by dot-ELISA and Western immunoblotting compared favourably with the CFT data. The present data utilizing dried blood on filter papers indicate that there is a high prevalence of anaplasmosis in those regions of Uganda surveyed; and confirm our observations and those of others that collecting blood on filter papers is a suitable technique for large scale screening and for seroepidemiological studies