Effects of the Sikle Cell (S) Gene on Serum Protein Profile

Total serum protein, albumin, total globulin levels, albumin/globulin ratio as well as the various globulin fractions were determined in 96 subjects, 39 are sickle cell (SS) disease subjects (steady state), and 30 are heterozygous sickle cell (AS) trait and 27 normal control subjects. The mean standard deviation of total protein was significantly higher (P< 0.05) in sickle cell disease when compared with heterozygous AS and normal controls. There was also significant difference (P<0.05) between AS and AA. The albumin level in sickle cell disease was significantly higher than in the other two groups. There was hyperglobuneamia observed in SS individuals with a mean ±SD of 32.6±10.0g/L when compared with AS and AA subjects with a mean ±SD of 30.8± 5.9g/L and 26.7±6.2g/L respectively. There also exist a significant difference between AS and AA (P <0.005). The Albumin/Globulin ratio is significantly lower (P <0.05) in SS than the other two groups but there was no significant difference (P>0.05) observed between AS and AA controls. The globulin fractions were observed to be higher in AS individuals except the gamma globulin which is higher in SS subjects. The hyperproteineamia as a result of the hyperglobulineamia is due to the globulin fraction present in the serum of SS individuals. The AS individuals are protected from various infectious disease conditions because of the high acute phase reactants and ß- globulin present in their serum as these has been observed to exert some immunioregulatory role

Effects of dietary protein quality and quantity on albino rat tissue serum protein ; erythrote fragility and bone mineral content

The study was to ascertain the effect of quality and quantity of dietary protein on body growth; organ cellular and cytoplasmic size; serum protein concentration and molecular weights; erythrocyte fragility and bone mineralisation. Diets containing 20; 17 and 15protein were fed to Sprague Dawley Albino rats weighing about 90g for 21 days. The 20protein diet was a commercial diet better in nutrient composition and quality than the diet containing 17 and 15protein formulated in our laboratory. At the end of 21 days; kidney; testes; brain; pancreas; spleen and long bones ere taken for analysis. Results showed that the diet containing 20protein supported higher body weight; liver and testes cell size (Protein/DNA and weight/DNA ratios) and cytoplasmic size (RNA/DNA ratio). The pancreas cell size; heart; brain; pancreas and spleen cytoplasmic size were not changed by dietary protein level. Plasma protein concentration and the molecular weights; percent bone cash and percent calcium in it were the same at the three levels of dietary protein. TCA soluble plasma amino acid concentration was higher at 20dietary protein. Erythrocyte fragility was higher but erythrocyte ghost protein was lower at dietary protein below 20

Effects of Dietary Protein Quality and Quantity on Albino Rat Tissue Serum Protein; Erythrocyte Fragility and Bone Mineral Content

The study was to ascertain the effect of quality and quantity of dietary protein on body growth; organ cellular and cytoplasmic size; serum protein concentration and molecular weights; erythrocyte fragility and bone mineralisation. Diets containing 20; 17 and 15protein were fed to Sprague Dawley Albino rats weighing about 90g for 21 days. The 20protein diet was a commercial diet better in nutrient composition and quality than the diet containing 17 and 15protein formulated in our laboratory. At the end of 21 days; kidney; testes; brain; pancreas; spleen and long bones ere taken for analysis. Results showed that the diet containing 20protein supported higher body weight; liver and testes cell size (Protein/DNA and weight/DNA ratios) and cytoplasmic size (RNA/DNA ratio). The pancreas cell size; heart; brain; pancreas and spleen cytoplasmic size were not changed by dietary protein level. Plasma protein concentration and the molecular weights; percent bone cash and percent calcium in it were the same at the three levels of dietary protein. TCA soluble plasma amino acid concentration was higher at 20 dietary protein. Erythrocyte fragility was higher but erythrocyte ghost protein was lower at dietary protein below 20

Maternal serum alpha fetoprotein screening test in routine antenatal care : Is it cost effective in Cameroon?

Screening test for maternal serum alpha feoto-protein (MS-AFP) in routine antenatal care is compulsory in most developed countries to detect neural tube defect (NTD). With the cost of suck a programme; there was a need to evaluate its need in our environment. A high risk clinic was choosen for the recruitment of the subjects to maximize the risk. Incidence of NTD could be considered to be low in Cameroon compared to countries where screening for MS-AFP is compulsory. Coupled with the low socio-economic status of our population and the lack of infrastructures; we could conclude that this screening test on a routine basis is not cost-effective