Investigation of rodent reservoirs of emerging pathogens in Côte d'Ivoire, West Africa

Background: One of the main health problems in West Africa remains upsurge of emerging pathogens. Ebola virus disease outbreak occurred in 2014 in Liberia, Guinea and Sierra Leone, Monkeypox virus in Nigeria in 2017 and most recently Lassa virus in Nigeria, Togo and Benin in 2018. These pathogens have animal reservoirs as vectors for transmission. Proper investigation of the pathogens in their rodent vectors could help reduce and manage their emergence and spread. Methodology: This study was conducted with an approval from the Côte d'Ivoire Bioethics Community. Small mammal trappings were carried out in 9 sites within three zones namely, peri-urban, peri-rural and protected areas. Liver, lung and kidney tissues from trapped small mammals were sampled in accordance with the recommended conditions of biosafety and bioethics. The organs were transported in liquid nitrogen to the laboratory. Molecular tests were used to detect pathogens. Orthopoxviruses and Monkeypox virus were detected in the organs by PCR using consensus primers targeting the virus surface membrane haemagglutinin (HA) genes, while Leptospira species were detected by PCR using primers targeting the rrs and lfb1 genes. Results: Out of 4930 night-traps, 256 (5.19%) small mammals were trapped including Crocidura, Rattus, Lophuromys, Praomys, Mus and Mastomys. Leptospira species were detected in 6 genera from 7 study sites and the infected small mammals accounted for 13.3%. Leptospira sp was detected mainly in the rodent vector genera Rattus (32.3%), Lophuromys (29.0%), and Praomys (16.1%). Three species of Leptospira were detected and Leptospira interrogans was the most common frequent species (74.2%). Monkeypox virus was not detected from studied small mammals. Conclusion: The initial data from our investigation indicates the presence of Leptospira sp in rodent vectors, Rattus, Lophuromys and Praomys, which are the potential small mammalian reservoirs of this pathogen in Cote d'Ivoire.

Bacterial reservoirs in a health care environment: case of the Douala general Hospital, Cameroon

Background:Health care acquired infections are a major cause of morbidity and mortality in hospitals. There is very little documentation of this important problem in our resource limited setting. The aim of our study was to identify bacterial reservoirs and microorganisms with the potential of nosocomial infections in our health care environment Methods:Bacteriological samples from the air; surfaces; equipment; personnel and patients from six units of the Douala General Hospital; Cameroon were collected for culture according to standardised collection; culture and germs identification techniques. of all collected samples were contaminated with pathogenic bacteria. These included 83.3 of air samples and 100; 54.1and 48.7of specimens respectively. The same bacteria found on the hands of nursing personnel were same as on both surfaces and equipment. The intensive care and neonatal units were the most contaminated with 100 positive cultures. The sterilization unit was bacteria free

Seroprevalence of Leptospira Check for this Species in other Resources Infection in Bats Roosting in Human Settlements in Morogoro Municipality in Tanzania

Leptospirosis is a neglected emerging infectious disease with high burden in Africa. Major reservoirs of leptospirosis are rodents and other small mammals. Studies of leptospirosis in bats and the extent to which they contribute to its transmission to humans and domestic animals in Tanzania are lacking. The objective of this study was to determine the prevalence of leptospirosis and local Leptospira check for this species in other resources serovars in bat colonies found around human settlements in ; Tanzania. In this study; mist nets were used to capture bats at Sokoine University of Agriculture main campus; whereas bats roosting on trees at Nunge A; Nunge B and Mwere primary school were captured manually. Leptospirosis was determined using the gold standard microscopic agglutination test for determination of Leptospira antibodies. Six live Leptospira serovars including local Leptospira serovar Sokoine; Kenya and Lora reported in rodents and domestic animals in Tanzania; and reference serovars Hebdomadis; Canicola and Pomona were used in the study. Leptospirosis prevalence was high in bats (19.4) and higher within colonies (27.3). Leptospira serovar Sokoine was more prevalent (19.4) compared to serovars Kenya (2.8) and Lora (2.8). Serovars Pomona; Canicola and Hebdomadis were not detected in bats. In conclusion; bats are potential reservoir and transmitter of Leptospira serovar Sokoine; hence should be prevented to access houses and roosting in human settlements to avoid public health risks. Routine diagnosis of human leptospirosis is needed in Tanzania as evidence show a high prevalence of Leptospira in reservoir hosts interacting with humans