ABSTRACT
Abstract: The effects of aqueous extract of Hibiscus sabdariffa calyces on haematology and pathological changes in some selected organs during experimental Trypanosoma congolense infection of rats were investigated. Three groups of rats were intraperitoneally infected with T. congolense (Karu stock). One group was administered with the aqueous extract and another given a solution of vitamin C in drinking water; the remaining infected group was left untreated. Data from these groups were compared with those of two groups of healthy rats, one of which was similarly treated with the aqueous extract. The experiment was terminated three weeks, post-infection (pi). The uninfected and infected rats administered the extract consumed the equivalent of 9.94 mg and 9.61 mg ascorbic acid / 100g / day during the experiment. Consumption of the extract significantly (p<0.01) retarded the rate of weight gain in both healthy and infected rats; even though the feed-intake was not significantly affected. After two weeks of infection the extract and vitamin C kept the parasitaemia significantly (p<0.01) lower than the untreated infected group. The anaemia in the untreated infected group was significantly (p<0.01) more severe than that of the corresponding extract- or vitamin-treated groups. Trypanosoma congolense infection caused significant (p<0.01) decreases in serum total proteins and albumin; serum and organ ascorbic acid as well as significant (p<0.01) elevation of serum alanine amino transferase levels in untreated rats. Consumption of the extract or vitamin C, however, prevented these diseaseinduced anomalies in the treated infected rats. Serum creatinine and urea levels were not affected by infection but the extract elevated these parameters significantly (p<0.01) above infection levels. It was concluded that consumption of the extract ameliorated the pathological changes in blood and organs of T. congolense-infected rats
Subject(s)
Anemia , Hibiscus , Plant Extracts , Trypanosoma congolenseABSTRACT
This study evaluated immunomodulatory properties and the sub-acute toxicity profile of two fractions of the aqueousalcoholic extract of the dried calyx of Hibiscus Sabdariffa in experimental animals. Immunomodulatory activity was evaluated using red blood cell-induced immunostimulation. The fractions were not found to be toxic after 7-day administration; though there was severe weight loss with the residual water-soluble fraction (RWSF) and weight gain with the ethyl acetate soluble fraction (EAC). The EAC exhibited a significant dose-dependent immunostimulation (p0.05)higher than thatobserved for levamisole (positive control). The residual water-soluble fraction exhibited immunostimulatory activity at 100mg/kg body weight. The two fractions caused a significant reduction in production of tissue necrosis factor - alpha and an increase in interleukin 10 (IL-10)
Subject(s)
Cytokines , Hibiscus , Plant ExtractsABSTRACT
Pour la prise en charge des infections urinaires et la cystite, les plantes médicinales constituent une source de nouvelles molécules à activité antimicrobienne économiquement accessibles pour faire face à l'apparition de phénomènes de résistance des germes aux antibiotiques. Les infections urinaires et la cystite sont très répandues et constituent une préoccupation importante de santé publique dans les pays en développement: Environ 50% des femmes développent au moins une fois une infection symptomatique des voies urinaires dans leur vie. L'infection urinaire est, par ordre de fréquence, la première des maladies infectieuses non épidémiques. Le but de notre étude est de trouver dans la pharmacopée malienne des plantes dotées de propriétés antibactériennes et antalgiques. Cinq recettes et leurs plantes utilisées en médecine traditionnelle pour la prise en charge des infections urinaires et de la cystite ont été sélectionnées, sur la base de résultats d'enquêtes ethnobotaniques. Des extraits des recettes et leurs plantes ont été testés pour leur activité antibactérienne sur des germes responsables des infections urinaires et de la cystite. Les extraits des recettes contenant Stylosanthes erecta P. Beauv (Fabaceae) ont démontré une activité antibactérienne contre les souches cliniques de Escherichia coli, responsable de 75 à 80 % des infections urinaires. Les mêmes extraits ont démontré une activité antalgique contre la douleur provoquée par l'acide acétique chez les souris. Les propriétés antibactériennes et antalgiques peuvent être bénéfiques dans la prise en charge des infections urinaires et de la cystite
Subject(s)
Anti-Infective Agents, Urinary , Cystitis , Hibiscus , Mali , Plants, Medicinal , Urinary Tract InfectionsABSTRACT
"In folk medicine; extracts of Hibiscus Sabdariffa (HS) are used in the treatment of several complaints including high blood pressure; liver diseases and fever. To the best of our knowledge; no documented evidence exists on the effect of inutero exposure to an aqueous extract of HS on postnatal growth. The purpose of the present study; therefore; was to investigate this. Three groups of pregnant rats were used for this study. Group C had tap water while groups A and B had 0.6g and 1.8g HS extract respectively in 100ml tap water to drink throughout pregnancy. All groups had normal rat chow ad libitum. On the day of birth; birth weights were recorded and two pups each from dams in groups A and B were substituted for 4 pups from dams in group C such that dams in group C had two pups each from groups A and B and at least two pups from its own litters to nurse. Thereafter; weights were recorded at 10days; 14days; 20days and 34 days postpartum.Results of the present study show a statistically significant (p0.05) growth reduction in groups A and B pups (exposed group) compared with group C (unexposed group) at 10 days through 20 days postpartum with no statistically significant growth reduction at 34 days postpartum. We conclude that inutero exposure to an aqueous extract of HS attenuates early postnatal growth in SD rats with a possible ""catch-up"" growth later in life."
Subject(s)
Animal Experimentation , Hibiscus , Pregnancy , RatsABSTRACT
"In folk medicine; extracts of Hibiscus Sabdariffa (HS) are used in the treatment of several complaints including high blood pressure; liver diseases and fever. To the best of our knowledge; no documented evidence exists on the effect of inutero exposure to an aqueous extract of HS on postnatal growth. The purpose of the present study; therefore; was to investigate this. Three groups of pregnant rats were used for this study. Group C had tap water while groups A and B had 0.6g and 1.8g HS extract respectively in 100ml tap water to drink throughout pregnancy. All groups had normal rat chow ad libitum. On the day of birth; birth weights were recorded and two pups each from dams in groups A and B were substituted for 4 pups from dams in group C such that dams in group C had two pups each from groups A and B and at least two pups from its own litters to nurse. Thereafter; weights were recorded at 10days; 14days; 20days and 34 days postpartum. Results of the present study show a statistically significant (p0.05) growth reduction in groups A and B pups (exposed group) compared with group C (unexposed group) at 10 days through 20 days postpartum with no statistically significant growth reduction at 34 days postpartum. We conclude that inutero exposure to an aqueous extract of HS attenuates early postnatal growth in SD rats with a possible ""catch-up"" growth later in life."
Subject(s)
Growth and Development , Hibiscus , Plant Extracts , Pregnancy , RatsABSTRACT
Ce travail a porte sur l'etude de 5 recettes traditionnelles utilisees dans le traitement des infections urinaires et de la cystite. Les objectifs fixes etaient de selectionner par un screening phytochimique et biologique parmi 5 recettes traditionnelles les plus interessantes en vue d'approfondir notre etude par des tests antibacteriens; hemostatiques (hemolytique) et antalgique sur ces dernieres. Les reactions en tube; la chromatographie sur couche mince ont montre la presence de nombreux composes susceptibles de manifester les activites recherchees. Les resultats des etudes scientifiques sur 2 recettes a 20a base de Stylosanthes erecta associe a Tamarindus indica ou a l'Hibiscus sabsdariffa confirment leur utilisation traditionnelle par leur activite antibacterienne sur Escherichia coli responsable de 75 a 80des infections urinaires; leur activite antalgique est comparable a celle du Paracetamol et de l'Indometacine dans nos conditions experimentales