Phytochemical analysis, antioxidant and anti-Candida albicans activities of Annona cherimola Mill. fruit pulp

Aims: The main objectives of the present study were to characterize the phytochemical profile of Annona cherimola Mill, to assess its antioxidant characteristics and its antifungal activity against Candida albicans. Methods and Material: Aqueous decoction, aqueous infusion, aqueous maceration, and methanolic maceration were screened for the presence of phytochemicals and to quantify content of phenolics, tannins, and flavonoids. Furthermore, antioxidant activity was assessed using DPPH and FRAP assays, as well the assessment of antifungal activity for the different extracts. Results: Results showed that phenols, tannins, flavonoids, and saponins were present in the four extracts. The aqueous maceration extract presented the highest total phenolic content (3.687 mg GAE/g of extract). Decoction extract showed the lowest phenolic content 2.504 mg GAE/g. Besides, infusion showed the most important total flavonoids content (2.567mg CE/g). The most relevant total antioxidant activity was found for decoction (lowest IC50 12.61 mg/ml AAE). The aqueous maceration exhibited the less antioxidant activity (IC50= 21.98 mg/ml AAE). The best scavenging activity was observed for decoction (IC50=7.27 mg/mL). All the extracts showed a reducing capacity. Infusion exhibited the best reducing power (EC50 = 11.29 mg/mL), compared to decoction (EC50 = 39.32 mg/mL). Regarding antifungal activity, at 100 and 200 µg/mL, decoction and methanolic maceration resulted in 6 and 9 mm inhibition zone, respectively. In addition, at higher doses (800 and 2000 µg/mL), inhibition zone increased in a dose dependent manner for all the extracts. Conclusions: Annona cherimola Mill. could be an important source of bioactive molecules with antioxidant and antifungal activities

Screening phytochimique de deux especes de plantes : crotalia retusa L (Papilionaceae) et hallea ciliata Aubrev et Pellegr. (Rubiaceae) recoltees au Gabon

Notre etude qui a porte sur la determination de la composition en substances chimiques organiques de deux especes vegetales : Crotalaria retusa et Hallea ciliata recoltees au Gabon a comporte : Un rappel sur les travaux anterieurs qui a permis d'obtenir des renseignements sur les utilisations traditionnelles de Hallea ciliata et de Crotalaria retusa (en plus de la toxicite de cette derniere) ;Des reactions de caracterisation qui ont permis d'identifier differents groupements chimiques que renferment ces deux especes qui sont : les alcaloides; les tanins; les flavonoides; les sterols et terpenes; les saponosides; les composes reducteurs; les mucilages; les derives anthraceniques; les heterosides cardiotoniques et les coumarines. Les trois derniers cites etant en quantites moins importantes.Une chromatographie sur couche mince des alcaloides totaux au cours delaquelle; apres revelation avec la reactif de Dragendorff; nous avons obtenu pour Crotalaria retusa un spot pour les feuilles et deux pour chacun des quatre autres organes etudies (fleurs; tiges; graines; racines). Et pour les ecorces de Hallea ciliata nous avons egalement obtenu deux spots