Your browser doesn't support javascript.
loading
Show: 20 | 50 | 100
Results 1 - 2 de 2
Filter
Add filters








Language
Year range
1.
Article in English | AIM | ID: biblio-1342398

ABSTRACT

Afr. J. Biomed. Res. Vol. 24 (May, 2021); 257- 263 Research Article Protective Effect of Alkaloid-rich Extract of Brimstone Tree (Morinda lucida) on Neurotoxicity in the Fruit-fly (Drosophila melanogaster) Model Nwanna E.E. Functional Food, Nutraceutical and Phytomedicine unit Department of Biochemistry, Federal University of Technology Akure PMB 704, Nigeria ABSTRACT Brimstone plant is one of the medicinal plants found in Nigeria used in fore-lore medicine with little scientific information on its alkaloid constituents. This study was aimed at investigating the effect of alkaloid-rich compounds from the plant in manganese- induced (MgCl2) neurotoxicity in the fruit fly. In addition, alkaloid compounds will be characterized using gas chromatograph coupled with flame ionization detector (GC-FID). Alkaloid-rich extract was prepared by solvent extraction method, fruit flies were pre-treated with the extract (0.5 ­ 1.0mg/ml) in a fortified diet before induction with MgCl2. The survival rate and negative geotaxis were observed. Thereafter, the activity of acetylcholinesterase (AChE) enzyme, antioxidantive potentials in in -vivo reactive oxygen species (ROS) thiobarbituric acid reactive species (TBARS), total thiol content, nitric oxide (NO*), hydroxyl oxide (OH*) scavenging ability, ferric reducing antioxidant property (FRAP) and 2, 2'-azino-bis (3-ethylbenzthiazoline-6- sulphonic acid) (ABTs*) radical scavenging ability were carried out on the flies' homogenate. Results obtained revealed that the alkaloid-rich extract regulated the neuro-protective activity of AchE, reduced the reactive oxygen species level in the induced flies with an increased in antioxidantive potential, higher survival rate and increases in the life span of the flies with 50% reduction in the mortality rate. The GC-MS showed a total of (1.55/100mg) of different plant-derived alkaloids such as dicentrine, atropine, aporphine. These alkaloids-rich compounds were found to have anti-oxidative, anti-nociceptive, anti-inflammatory and anti- cholinergic activities. In conclusion, this study suggests that alkaloids from brimstone plant could be the reason for the observed biological activities for the prevention of neuronal related complications.


Subject(s)
Humans , Sulfur , Neurotoxicity Syndromes , Acetylcholinesterase , Drosophila Proteins , Alkaloids
2.
Article in English | AIM | ID: biblio-1265829

ABSTRACT

The assessment of heavy metals (lead and mercury) and by-product (sulphur) on fried food sold by roadside food vendors in Calabar; Nigeria were investigated. The food samples which were covered and uncovered after frying were obtained randomly from 20 street food vendors. The food samples were obtained from 10 busy roads and 10 non- busy roads in Calabar; Nigeria. The busy roads were those roads that recorded more than 20 cars per minute; while the non-busy roads recorded less than 10 cars per minute. The result showed that the busy roads had significant (P0.05) higher depositions of heavy metals and by-products than the non-busy roads. Lead deposits on the food samples was significantly (P0.05) higher than the amount of mercury and significantly (P0.05) higher than the amount of sulphur deposit. The amount of sulphur deposit on the food samples was significantly higher (P0.05) than mecury. The food samples that was uncovered after frying had a significantly higher (P0.05) deposit of heavy metal and by-product than the food samples that were covered after frying. This result suggest that fried foods vended in calabar by the road-side have a serious public health concern


Subject(s)
Commerce , Food , Food Contamination , Lead , Mercury , Sulfur
SELECTION OF CITATIONS
SEARCH DETAIL