Antibiotic resistance and virulence properties in escherichia coli strains from cases of urinary tract infections

This study determined E. coli resistance to commonly used antibiotics together with their virulence properties in Ile-Ife; Nigeria. A total of 137 E. coli isolates from cases of urinary tract infection were tested for their sensitivity to commonly used antibiotics and possession of virulence factors using standard methods. Their ability to transfer resistance was also determined. The isolates demonstrated a high and widespread resistance (51.1 to 94.3 ) to all the antibiotics used except Nitrofurantoin (7.3 ). A total of 50 (36.5 ) of the isolates were resistant to 10 of the eleven antibiotics employed. Sixty three per cent (63 ) of the 107 trimethoprim resistant E. coli transferred their resistances while amoxicillin; gentamycin; augmentin; tetracycline and erythromycin were co-transferred with trimethoprim. Fifty one (37.2 ) of these multi-resistant isolates possessed one or more virulent factors. The study concluded that urinary tract infection due to E. coli in Ile-Ife may be difficult to treat empirically except with nitrofurantoin; due to high resistance to commonly used antibiotics. It is imperative that culture and susceptibility tests be carried out on infecting pathogen prior to treatment; in order to avoid treatment failure and reduce selective pressure that could result in the spread of uropathogenic E. coli in the environment

Suspected urinary tract infection : identification of micro-organisms and sensitivity to antibiotics in Seychelles

Urinary tract infection (UTI) is commonly diagnosed and may require antibiotic treatment. To identify micro-organisms found in suspected UTI and micro-organism sensitivity to antibiotics; a study was conducted on 792 consecutive urine samples collected in hospitals and clinics in Seychelles. Among the 792 samples; 70.8 per cent showed no growth and only 6.3 per cent showed growth for specific organisms; while the remaining 22.9 per cent showed mixed growth (likely to indicate contamination). E.coli was the micro-organism most often identified while Klebsiella; Proteus and Pseudomonas were found in a few cases. As much as 78.6 per cent of coliform organisms were resistant to Ampicillin and 54.8 per cent were resistant to Septrin while 75 per cent were sensitive to Gentamicin; Nitrofurantoin and Nalidixic acid. Results of this study suggest that routine urine culture on suspicion of uncomplicated UTI is unnecessary (however clinical practice requires that urine culture is done in complicated or recurrent UTI). A test to detect leucocytes in a midstream sample of urine (e.g.; Combur 9) is an alternative; simple and inexpensive way to identify the presence of bacteriuria in patients with uncomplicated UTI. In patients testing positive for leucocytes (and in all patients with complicated UTI); culture and antibiotic sensitivity should be carried out and a Nalidixic acid course can be a first choice treatment

Les infections urinaires de l'enfant en pratique hospitaliere a Lome : experience du CHU Campus

A partir d'une etude prospective sur une periode de 9 mois chez 225 enfants; les auteurs rapportent leur experience sur la prevalence de l'infection urinaire au cours des syndromes infectieux et decrivent les principaux germes en cause ainsi que leur sensibilite a l'antibiogramme. Ils attirent l'attention sur la symptomatologie polymorphe et souvent non specifique de l'infection urinaire chez les tout-petits et la frequence de l'association infection urinaire et goutte epaisse positive

Antibiotic susceptibility of escherichia coli isolates from inpatients with urinary tract infections in hospitals in Addis Ababa and Stockholm

A high level of antimicrobial resistance of bacteria has been detected at the Tikur Anbessa Hospital (TAH), Addis Ababa, for many years. In contrast, at the Karolinska Hospital (KH), Stockholm, the level of resistance is low. Reported are the results of an investigation of the correlation between antibiotic usage and the antimicrobial resistance rates of Escherichia coli isolates from patients with urinary tract infections in these hospitals. At TAH the strains of E. coli isolated were considerably more resistant to all seven antibiotics tested. The level of multiresistance was 63% at TAH and 7% at KH. There were no significant differences in the total amount of antibiotics used in the two hospitals, except for antituberculosis agents. The strain biotypes and antibiograms, together with the length of patients' hospitalization before a positive urine culture was obtained, suggest that the majority of the strains from TAH were of nosocomial origin