Sero-molecular Epidemiology of Hepatitis E Virus in Blood Donors, Gezira State, Sudan: A Cross-sectional Study

Background: Hepatitis E virus (HEV) is a hepatotropic pathogen that causes significant morbidity and mortality in humans. It is an important causative agent of viral hepatitis outbreaks. This study investigates the serological and molecular prevalence of HEV in blood donors attending the Central Blood Bank in Wad Medani City in Gezira State, Sudan. Methods: The study adopted a cross-sectional descriptive design. A structured questionnaire was used to collect data concerning demographic information and risk factors associated with HEV transmission. All enrolled participants (N = 300) were screened for HEV IgG antibodies using commercial ELISA kits, then strong positive samples (N = 84) were selected and rescreened for HEV IgM and HEV RNA by RT PCR. SPSS version 24.0 was used for analysis. Results: Out of 300 male participants, 36.3% (109/300) were positive for HEV IgG. However, only one participant was IgM positive, while the HEV RNA was negative. The highest prevalence rates of the virus were 42 (44.6%) among the age group of 31­40 years, 20 (48.8%) in those who consumed food from outside, 13 (50%) in three to four multiple blood donations, and 5 (62.5%) in those who consumed water from the river source. A significant association of HEV IgG prevalence concerning the occupation of the participants being students or farmers was detected using univariate and multivariate analysis (P-value = 0.007).

Prevalence of hepatitis B virus infections among blood donors in Federal Capital Territory, Abuja, Nigeria

Background: Transfusion of hepatitis B virus (HBV) infected blood represents a major but avoidable means of HBV transmission, which unfortunately still account for millions of global HBV infections annually. Method: This study determined the prevalence of HBV infection among 550 blood donors aged 18 to 60 years from selected hospitals and blood transfusion centres within the Federal Capital Territory, Nigeria, using hepatitis B surface antigen (HBsAg) rapid diagnostic test (RDT) kit and Enzyme linked Immuno-sorbent Assay (ELISA). Representative positive and negative samples for RDT and ELISA were tested by both conventional and real-time polymerase chain reaction (PCR) assay Results: Forty nine (8.9%) and 14 (2.8%) out of the 550 blood donors tested positive for HBsAg with the RDT and ELISA respectively. Replacement donors had higher prevalence rate of the HBV infection than voluntary donors. The highest prevalence of HBV infection was recorded among the 30­39 year age group. The difference in the rate of infection between the males and the females was not statistically significant (p>0.05). A perfect agreement between RDT and PCR and fair agreement between ELISA and PCR were observed. Conclusion: This study report a high prevalence of hepatitis B virus infections among blood donors in Abuja, Nigeria which underscores the need for proper screening of blood for transfusion to completely eliminate the incidence of transfusion transmitted HBV infections

Knowledge of blood donation among adults in north-central Nigeria

Background: About half of the population in Nigeria is medically fit for blood donation but only four in one thousand are voluntary donors. The low level of blood donation has been attributed to poor knowledge, misconceptions, myths, bias, poverty, fear, malnutrition among the population. Therefore, this study assessed the knowledge of blood donation among adults in two selected North Central States of Nigeria.Methods: It was a descriptive cross-sectional study. A total of 3104 respondents comprising of adults between 18 and 60 years were involved in the study. A multistage sampling technique was used and the research tool was interviewer-administered questionnaire. The data generated were entered into the computer and subjected to appropriate statistical analysis using EPI INFO computer software package (version 3.5.3). Pearson Chi Square (χ2) was used to test statistical significance and p-value was set at < 0.05.Results: Majority of the respondents 2565 (82.5%) knew that blood donation save lives. More than three-quarters, 2468 (79.5%), knew where to go for voluntary blood donation. About one-third, (37.1%), demonstrated good knowledge of voluntary blood donation. Older respondents (>60 years) had poor knowledge of blood transfusion compared with younger age groups (p<0.001). Respondents' occupation and educational status were significantly associated with knowledge of blood transfusion (p<0.001)Conclusion: Periodic awareness programme on voluntary blood donation in rural and urban areas across Nigeria is needed. In addition, sensitization of the informal sector on the significance of non-remunerated voluntary blood donation should be given priority

Knowledge and attitude of undergraduates towards blood donation: a cross sectional study of the University of Benin, Benin City

Background: Blood is an essential but scarce therapeutic element. The youth constitute an important age group that can contribute significantly to donor blood availability in health institutions. Their knowledge and attitude towards blood donation may influence their disposition to blood donation practices. Objective: The study seeks to determine the knowledge, attitude and blood donation practices among undergraduate students of the University of Benin. Methodolgy: This is a cross sectional study, conducted at the University of Benin, Benin City. Two hundred pretested questionnaires on knowledge, attitude and practice of blood donation were distributed among consenting undergraduate students across different faculties of the university. Data was analyzed with the statistical package for social science (SPSS IBM) version 20. Result: A total of 155 (79.5%) males and 40 (20.5%) females (M:F of approximately 3.8:1) completed the questionnaires. Their modal age group was 16 ­ 20 years. Sixty eight (34.9%) had good knowledge of blood donation process, 43 (22.1%) had average knowledge and 84 (43.0%) had poor knowledge. Repeat donation rate was 11.3% among the respondent. The practice of blood donation was significantly associated with good knowledge of blood donation process (p = 0.001). Blood donation was higher in those between the age groups of 21 ­ 25 years. Conclusion: Good knowledge of blood transfusion is below average. Blood donation practices is low among undergraduate students of the University of Benin and there is high disposition to family replacement donation

Iron Deficiency in Frequent and First Time Female Blood Donors

Background: Blood donation has a marked influence on the body iron stores especially in female blood donors . Iron deficiency anaemia is an important limiting factor for the number of donations in female regular blood donors. Aim of the study: This study was conducted to evaluate the frequency of iron deficiency and relevant factors in frequent and first time female blood donors at Casablanca blood transfusion centre; Morocco. Methods: Between November 2005 and April 2006; twenty-one female first time and twenty-one frequent female blood donors were selected randomly. In frequent blood donors; only females with at least 10 donations were included. Haemoglobin concentration; serum ferritin; serum iron and total transferrine binding capacity were measured and analysed. Results: The results of haemoglobin concentration; serum ferritin; serum iron were significant lower in frequent female blood donors when compared with the results of same parameters in first time female blood donors. The results show that the frequency of iron deficiency in frequent female blood donors is 43and in the first time female blood donors is 14. Conclusion: Iron deficiency is very common in regular female blood donors at Casablanca's transfusion centre. Frequent blood donation has marked influence on the body iron stores in frequent female blood donors. It is therefore recommended that blood transfusion centres focused on maintaining iron balance by measuring serum ferritin and total transferrine binding capacity in frequent female blood donors .They have also to educate the donors about iron supplementation and yearly ferritin checking

The Seroprevalence of Cytomegalovirus Antibodies among Prospective Blood Donors in Jos

"Background:Human cytomegalovirus; otherwise called human herpes virus type 5; is a significant cause of morbidity and mortality in pregnancy; and among immunocompromised patients like recipients of organ transplants. Cytomegalovirus is transmissible via blood transfusion; among other parenteral routes. This study therefore aims at establishing the seroprevalence of CMV antibodies among prospective blood donors in Jos. This is with a view to making recommendations on donor screening and transfusion protocols among susceptible patients. Methods: A total of 200 prospective blood donors were recruited into the study. Screening for CMV antibodies was done using ELISA kit; manufactured by ""DIALAB""; Austria (www.dialb.at) Results: Of the 200 prospective blood donors analysed; 184 donors were found to be positive for cytomegalovirus antibodies; representing a prevalence rate of 92.The peak age prevalence was in the 25-29 years age range; representing 0.4.Cytomegalovirus prevalence was lowest in the 15-19 years age range and above 50 years(1.6each). Conclusion: A cytomegalovirus antibody prevalence rate of 92indicates that screening for CMV should be included in screening algorithm for potentially susceptible recipients of blood and its products. Non infected susceptible persons should be transfused CMV negative or leucocyte depleted blood and blood products."

La sécurite sanguine

Dans ce numero on nous montre que la meilleure strategie pour avoir du sang non contamine consiste a encourager les personnes qui presentent un faible risque de contamination par le VIH a donner leur sang et a les conseiller sur la necessite d'etre des donneurs responsables. Il semble que cette strategie presente un double avantage : d'une part; la proportion de sang contamine est moindre dans les collectes se qui diminue les risques pour les receveurs et; d'autres part l'exemple des donneurs reguliers montre le role central qu'ils jouent dans l'education relative au don du sang et a la prevention du virus