Fluorescence microscopy for the diagnosis of smear-negative pulmonary tuberculosis in Ethiopia
Afr. j. lab. med. (Online)
; 9(1): 1-6, ilus
Article
in English
| AIM
| ID: biblio-1257337
Responsible library:
CG1.1
ABSTRACT
Objective:
This study aimed to evaluate the diagnostic performance of LED-FM for smear-negative pulmonary tuberculosis in Ethiopia.Methods:
A total of 194 adult patients with a cough lasting for more than two weeks, and who had three direct smear-negative sputum tests for Mycobacterium tuberculosis by Ziehl-Neelsen light microscopy, were included. All direct Ziehl-Neelsen-stained smear-negative sputum samples were cultured and were also visualised by LED-FM. Smears for LED-FM were performed from bleach-concentrated sputum sediment. The diagnostic performance of the LED-FM was compared to the culture method (the reference standard).Results:
Of the 194 smear-negative sputum specimens analysed, 28 (14.4%) were culture-positive and 21 (10.8%) were LED-FM-positive for M. tuberculosis. However, only 11 of the 21 (52.4%) LED-FM-positive patients had a confirmed tuberculosis diagnosis by culture. Light-emitting diode fluorescence microscopy (FM) had a sensitivity of 39.3% (95% confidence interval 21.257.4) and specificity of 93.9% (95% confidence interval 90.497.6). Ten LED-FM-positive specimens were culture-negative, and all of these specimens had scanty grading (119 bacilli per 40 fields on LED-FM).Conclusion:
This study showed that implementation of LED-FM on bleach pre-treated and concentrated sputum can significantly improve the diagnosis of smear-negative pulmonary tuberculosis. However, all scanty grade, positive smears by LED-FM need to be confirmed by reference culturemethod:
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Index:
AIM (Africa)
Main subject:
Sputum
/
Bioprosthesis
/
Ethiopia
/
Lasers, Semiconductor
/
Microscopy, Fluorescence
Country/Region as subject:
Africa
Language:
English
Journal:
Afr. j. lab. med. (Online)
Type:
Article
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