In vitro Heparin Precipitation in the Plasma of Euthyroid women with Recurrent Miscarriage and Unexplained Infertility

ABSTRACT

OBJECTIVE: Women with elevated Lp(a); who are susceptible to atherosclerosis; get to reduce; their cardiovascular disease by in-vivo administration of low dose heparin. And history of recurrent miscarriage associated with auto antibodies have had a high rate of life births in subsequent pregnancies when they were treated with low dose aspirin together with low dose heparin. An in-vitro assay thus provide basis for the intricate interaction.DESIGN: Two hundred and ten (210) healthy volunteered Euthyroid women were used for this study. They were aged between 21-35 years; 90 Women served as test groups and 120 women served as control. Antimicrosomal autoantibody (Anti-TPO) ELISA assay was determined. The plasma treatment at the different PH with heparin and aspirin in physiological buffers were carried out.RESULT: At PH5.0 (i.e. the Uterus physiological PH) using 100i.u/ml heparin in 0.2M acetate buffer yielded 70 precipitate compared to treatment with 250i.u/ml heparin in 0.2M buffer that yielded 40 precipitate while 100-i.u/ml heparin with 80mg aspirin treatment yielded 65 precipitate in the test group samples that were positive to Anti-TPO. Whereas in the 0.2ml acetate buffer alone (without heparin and aspirin) no precipitate was formed; compared to the precipitate formed with plasma of the control women yielding 15 using 100i.u/ml heparin ;5with 250i.u/ml heparin and 12 100-i.u/ml heparin with 80mg aspirin treatment respectively.At PH 4.6 (i.e blood physiological PH ) for the various treatment showed there were increases in percentage precipitate; more in the subjects than in the control groups. In the unexplained and spontaneous abortion; groups with the treatment of 100iu/ml Heparin in 0.2M acetate buffer gave highest precipitate compared to other treatment in in-vitro study. CONCLUSION: This study has further supported that Heparin in addition to its anticoagulant action; may act to reduce fetal loss by binding to antimicrosomal; (anti-phospholipids) antibodies; thereby protecting the trophoblast phospholipids attack; and thereby promote successful implantation in early pregnancy