Study of cloned gene-related protein Vp2 of infectious bursal disease virus-induced apoptosis in human lymphoma B cells in vitro

Apoptosis or programmed cell death [PCD] is an important mechanism in both development and homeostasis in adult tissue for the removal of superfluous cells, while its induction is an effective therapeutic approach for cancer. The aim of the present study was to evaluate the effect of cloned gene-related protein Vp2 of infectious bursal disease virus in human lymphoma B cells. In present study after cloning the Vp2 gene in Pichia pastoris system, the Vp2 protein was expressed. Cellular vital capacity was determined by MTT. Then effect of Vp2 protein in human lymphoma B cells was examined by Hoechst staining and flowcytometry techniques, respectively. During MTT, human lymphoma B cell lines revealed to have a meaningful apoptosis at 1 micro g and 5micro g protein concentrations when compared with controls [p<0.01]. Apoptotic bodies appeared by Hoechst staining apoptosis was induced suitably after 48 hours by flowcytometry assay. The present study is the first study that has revealed the gene-related protein Vp2 induced apoptosis in human lymphoma B cells in vitro

Antisense RNA to the type I insulin-like growth factor receptor reversed the transformed phenotype of PC-3 human prostate cancer cell line in vitro

The insulin-like growth factor I receptor [IGF-IR] plays an essential role in the establishment and maintenance of transformed phenotype. Interference with the IGF- IR pathway by antisense causes reversal of the transformed phenotype in many rodent and human tumor cell lines. We stably transfected the PC-3 human prostate cancer cell line with an IGF-IR antisense RNA expression plasmid. The number of lGF-I receptors on the antisense-transfected PC-3 cells was reduced by 40.2% relative to the control-transfected cells. The transfected cells maintained their high expression of IGF-IR antisense RNA for up to one year in selective medium. The reduction in the expression of IGF-IR had no effect on the cell growth in monolayer. The clonogenicity of antisense-transfected cells was 24.7% of the clonogenicity of control-transfected cells in soft agar. There was a good correlation between IGF-IR level and inhibition of transformation in soft agar. These results indicate that reduction of IGF-IR by antisense RNA can reverse the transformed phenotype of human prostate cancer cells