Study of toxicity and haemagglutinating activitiy of a purified intracellular toxin and extracellular crude toxin isolated from pseudomonas aeruginosa

The action of purified intracellular toxin [PIT] from Pseudomonas aeruginosa was examined in brine shrimp lethality bioassay. The LC50 of the PIT was calculated to be 25 microg/ml. The PIT agglutinated both albino rat and rabbit erythrocytes more potently than did extracellular crude toxin [ECT]. Galactose and Dmannose, however inhibited the agglutination property of PIT and ECT respectively. Intradermal injection of PIT caused changes on the tissues of rabbit skin at a lower dose than that of ECT

Evaluation of twenty four new Gossypium hirsutum strains for growth, yield, fibre quality and CICuV ressistance under environment of Punjab, Pakistan

Twenty four new strains were tested for their yield, quality and Leaf Curl Virus [ClCuV] resistance. NIAB-111/S, NIAB-98 and NIAB-999 were significantly high fruit bearing varieties at 90 days completing 59.54, 48.26 and 46.00% fruiting respectively. Seed cotton yield of VH-142 was highest with 5417 kgha-1 and 52 per plant boll bearing, DNH-57 and NIAB-999 remained second and third with 5234 and 5095 kg ha-1 seed cotton yield, respectively. BH-160 and CRIS-467 were found to be second and third highest boll bearing varieties with 50.97 and 40.20 per plant average bolls respectively. Plant height in CRIS-467, MNH-642 and SLH-224 was significantly higher in comparison to other varieties.NIAB-98, CIM-499 and CIM-506 were found short stature varieties in comparison with other but out of these NIAB-98 and CIM-506 were in high yielding position and CIM-499 was found medium yielder variety. GOT% of MNH-642 remained highest having value of 45.00%. While GOT%. of NIBGE-1, CRIS-168 and CRIS-467 was at lowest position with 35.73, 35.89 and 36.62% respectively. NIAB-111, CIM-499 and BH-160 were at first position in terms of fibre fineness with micronaire values 3.98, 4.00 and 4.07 micro g/inch having fibre length 28.53, 31.38 and 30.23 mm respectively. Out of 26 varieties, 16 varieties resulted maturity index in the range of 85.03% and 90.30% with highest maturity index in case of NIBGE-1 [i.e. 90.30%] followed by NIAB-999 and MNH-642 with 89.55 and 85.5% respectively.CRIS-168, CRIS-468 and CRIS-467 were found viral susceptible

Megacystis-microcolon-intestinal hypoperistalsis syndrome - report of a very rare pathology in a neonate

We are reporting a baby who presented with features of neonatal intestinal obstruction, with an x-ray picture suggestive of hold up at the duodenal level. On laparotomy he had malrotation of the gut which was corrected. He also had a dilated urinary bladder, which later on was treated with vasicostomy. The baby did not move his bowel inspite of no mechanical obstruction and even bypass procedure [gastrojejunostomy]. She had all the features of Megacystis-Microcolon-Intestinal Hypoperistalsis Syndrome [MMIHS]. MMIHS [also called hollow viscus myopathy] is a neonatal condition causing a severe form of pseudo [functional] intestinal obstruction. This is an autosomal recessive disorder more common in females. The various components of the syndrome are dilated urinary bladder, decreased motility of the gut, microcolon, intestinal malrotation and lax abdominal musculature. All these components of the syndrome were found in our case

Congenital pyloric atresia [CPA]: report of two cases, with review of literature

We are reporting two neonates with isolated pyloric atresia, with an objective to emphasize the importance of considering this rare condition in the differential diagnosis of upper intestinal atresias. Both had non-bilious vomiting and epigastric fullness. X-ray showed a dilated gastric shadow with no evidence of gas in the rest of the abdomen. On exploration they had pyloric atresia which was corrected with gastroduodenostomy. Congenital pyloric atresia is a rare condition, which presents with features of gastric outlet obstruction. It can be diagnosed antenatally but the picture can mimic other conditions. Epidermolysis bullosa has a strong association with it and require skin biopsy for diagnosis