[Effects of dietary fat sources on histological structure of testis in Zandi rams]

To investigate the effects of dietary fat sources on histological structures of testis in Zandi rams. Field trial on the basis of completely randomized design [CRD]. Eight 2.5 years old mature Zandi rams. Four experimental feed with different fat sources were used in four groups: without fat supplementation [control or diet 1], supplementation with 4% calcium soaps of fatty acids from tallow [diet 2], supplementation with 4% calcium soaps of fatty acids from soybean oil [diet3] and supplementation with 2% calcium soaps of fatty acids from tallow plus 2% calcified fatty acids from soybean oil [diet 4]. Values of Seminiferous tubule diameter, number of leydig cells, sertoli cells, spermatogonia, primary spermatocyte and spermatid were determined. Values of testis histological structures were compared by Duncan procedure with GLM. Results showed that ram's reproduction performance and testis histological structures were affected by nutritional conditions of animal [p<0.05]. Moreover, source of dietary fatty acids had significant effect on testis histological structures. Using soybean oil resulted in a significant increase in the seminiferous tubule diameter, seminiferous tubule lumen diameter and number of leydig cell, sertoli cell, spermatogonia and primary spermatocyte [p<0.05]

[Effects of different supplemental fats to flushing diet on reprodution of Zandi Ewes]

To determine the effects of adding of different supplemental fats to flushing diet on reproductive parameters in Iranian Zandi fat-tailed ewes. Randomized complete block. Fifty two Six- year- old Zandi ewes. Four experimental diets: without supplemental fat [1], containing 4.5% calcium salts of fatty acids from tallow [2], containing 4.5% calcium salts of fatty acids from soybean oil [3] and containing 2.25% calcium salts of fatty acids from tallow plus 2.25% calcium salts of fatty acids from soybean oil [4] were tested. After laparascopy, follicles [3 mm diameter] and corpus luteums [CL] on both ovaries were counted. Number of CLs were designated as ovulation rate [OR] index. At lambing number, weight and sex of lambs and lambing date of ewes were recorded. Analysis of variance was done by general linear model procedure of the SAS. OR in group 3 was higher than the other ones [p<0.05]. Number of follicles in group 2 were lower than the other groups [p<0.05]. Pregnancy rate from first, total of two and three first periods in group 3 were higher than the other groups [p<0.05]. Pregnancy rate from first and total of two first service periods in groups 2 and 4 were higher than group 1 [p<0.05]. Lambing rate and lamb crop from each of the three service periods were highest in group 3 and lowest in group 1[ p<0.05]. Twining rate from the first service period in group 3 was higher than, the other groups and in groups 2 and 4 was higher than group 1 [p<0.05]. Twining rate from total of two first service periods in groups 3 and 4 was higher than group 1 [p<0.05]. Fat supplementation especially from rich sources of unsaturated fatty acids to flushing diet had positive effect on the OR and reproduction performance of ewes

In vitro maturation of dromedary camel oocytes in Hamsvs F10 medium supplemented with different concentrations of fetal bovine serum

To mature dromedary camel oocytes for using them in an IVF system. Design: Interventional study. Ovaries from dromedary camels in local slaughterhouses. Removing varies from camels in a local slaughterhouse, carrying them to the laboratory in warm saline solution, aspiration of follicles, isolation and transferring of oocytes into TCM-199 and Ham's F10 supplemented with 0-10% heat inactivated fetal bovine serum [FBS], culturing oocytes for up to 24h in a COz incubator. After culture oocytes were denuded and put into PBS containing 0.1% hyaluronidase and passing through a fine pipette. Oocytes were then mounted onto slide glass and fixed and stained for evidence of maturation. ANOVA and when a significance different was seen, Duncan's Multiple Range Test. When oocytes from fresh ovaries were culture in Ham's F10 without protein, only 17.65% of them reached to MIL However, significantly [P<0.05] higher oocytes reached to Mil in 5 and 10% PCS [36.84% and 33.33% for 5 and 10% FBS respectively], which were not dose dependent. When cool stored ovaries were used for oocyte maturation, 14.54% of oocytes reached to MIL In protein-free medium However, significantly [P<0.05] higher oocytes reached to Mil in 5 and 10% PCS [25.86% and 33.33% for 5 and 10% FBS respectively]. Although increasing the protein increased the maturation rates, the difference was not significant. Under the present condition it seems that cool stored ovaries could be used for in vitro maturation of camel oocytes

[Experimental study on efficacy of supplemental organic and inorganic chromium in feeding of Shal fattening lambs]

Objective: Evaluating the differences in performance traits and carcass composition in Shal fattening lambs fed organic or inorganic chromium [Cr]

Design: Experimental study

Animals: Seventy fat-tailed ram lambs of Shal breed with an average body weight [BW] of 37.0 +/- 2.8 kg, randomly partitioned in seven groups

Procedure: Feeding a 60.5% barley-based diet [12.2% crude protein] supplemented with 0 [control], 200, 600, or 1000 ig/kg dry matter of Cr from chromium nicotinate [CrNic] and 200, 600, or 1000 ig/kg dry matter of Cr from chromium chloride [CrC13] to individually penned lambs, measuring dry matter intake [DMI] and average daily gain [ADG] through wk 12, and slaughtering lambs and determining carcass characteristics at the end of wk 15 of the experiment

Statistical analysis: Analysis of covariance for studying treatment differences and orthogonal coefficients for contrasting treatment groups

Results: Addition of 600 or 1000 ig/kg dietary dry matter of Cr from CrNic decreased ratio of DMI to% BW [P<0.04] but did not affect wk 6 and 12 body weights, ADG, daily DMI and ADG: DMI ratio. Backfat thickness increased by Cr [P<0.04]. Addition of 1000 ig/kg dry matter of Cr from CrNic increased loin-eye area [P<0.02] and muscle weight of right side carcass [P<0.04]. Percentages of water, protein, lipid, or ash in carcass muscle were not affected by Cr supplements, whereas weights of protein and ash in carcass muscle increased by Cr with more positive effect of 1000 ig/kg dry matter of Cr from CrNic [P<0.03]

Conclusion: The results of this study suggest that supplementation of chromium nicotinate may be beneficial for increasing lean tissue of Shal fattening lambs