Comparison of embryo quality cultured in Ham's F-10+%10 maternal serum and GI[III]

Appropriate culture media and environment for embryonic cell growth considered to be major factors for generation of an embryo in vitro. In this study two embryonic growth condition of Glm and Ham's F-10 + Maternal Serum [MS]%10 were compared. This investigation was prospectively performed on 100 infertile couples that were treated by IVF-iCSL The participants were divided into two equal groups. Ovules obtained from first group were treated with Ham's F[10] + Maternal Serum%10 culture media and the second group with Gl[III]. The variables affecting ovule and embryonic growth were measured, and collected data were analyzed by SPSS. The mean of age for tested ladies was 28.01 and the mean for duration of infertility was 6.87 years. The number of pronucleous with moderate quality were 2.14 in-group I and 3.22 in-group 11 [P=0.023]. The number of embryo with three cells were 1.26 and 0.54 and for grade B embryonic cell were 1.18 and 1.78 and grade C embryonic cell were 1.08 and 0.56 in group I and II respectively [p=0.29]. The mean of grade B blastomer in GI[III] media was more than Ham's F[10]+ Maternal Serum 10%. Compairing the means; the mean of grade B blastomers in Glm media was shown to be more than Ham's F[10] + Maternal Serum 10%. All of the findings showed that Gl[III] cultural condition is more effective than Ham's F[10] and the maternal serum as a supplement has no considerable impact

Effect of xylocaine on human normal sperm motility

Infertility as an obvious problem causes different social problems in family. One of the most common methods to solve the problem of infertile couples with azoospermia is capture via testis or epididymo biopsy. Use of anesthetic drugs is unavoidable for pain relief. Since these drugs can cause the reduction of sperm motility so it is necessary to determine the concentration of drugs that used for topical anesthetic. In this experimental study, semen samples of 30 men were studied. Post swim up samples [65ml] in Ham's F10 culture were put in six separated tubes and 10% of semen was mixed with xylocaine [20%, 40%, 60%, 80% and 100%]. Sample one was considered as control group and sperm motility and grading at 3 different times [0, 2, and 24 hours] were recorded. Data was statistically analyzed. Mean of sperm motility in control group was 63.20 +/- 5.31 and in test group decreased from 45.50 +/- 11.54 [xylocaine 20%] to 4.9 +/- 5.72 [xylocaine 100%] [p= 0.000]. Prolonged contact causes more decrease in motility and grading of sperm [p=0.001]. Increase of xylocaine concentration caused a decrease in sperm motility, so the effect of xylocaine on sperm parameters depends on dosage and duration of contact

[ in-vitro effects of Hinosan and Diazinon on human sperm parameters]

Hinosan and Diazinon are organophosphorate pesticides with contacting and penetrating effect that are used in agriculture against rice blast. Therefore, with regard to high use of these pesticides in the north of Iran, their harmful effects and high rates of infertility that are increasing every year in Mazandaran province. This study was performed to determine the effects of Hinosan and Diazinon on sperm parameters. This experimental study was performed on sperm samples of subjects who referred for semen fluid analysis [SFA]. The sperms were affected by Hinosan and Diazinon [0.001 and 0.0001 concentration]. The sperm number, motility and grading at the times of 0, 1, 2 and 4 were recorded. Data were analyzed by repeated measurement and compared with normal condition. Hinosan and Diazinon can reduce sperm motility by time [P<0.05], but the increase of dose had not significant effect on sperm motility decreasing. During the study, changes in increasing of grade I and grade II was statistically significant [P<0.05], but these changes were not significant in grade III. According to the findings, Hinosan and Diazinon can exert their reducing effects on number and motility of sperm as a time dependent profile. These effects are not dependent to the dose of the materials