Spectrophotometric methods in conjunction with thin layer chromatography applied for determination of sugars content in Egyptian honey and glucose syrup

A study was performed to choose the best solvent developer and adsorbent for separation of a sugar mixture composed of glucose, fructose maltose, sucrose and ribose. Thin layer chromatographic separation of the mixture is coupled with spectrophotometric method for its determination as anthrone method, phenol-sulfuric acid method and sulfuric acid method followed by application on the liquid glucose and Egyptian honey to determine qualitatively and quantitatively the content of the sugar in both of them. The solvent developer methanol, chloroform, acetone, water in ratio [2:2:12:1] and ethylacetate, acetic acid, water in ratio [6:3:2] are suitable for separation of the five sugar. On applying the spectrophotometric methods, anthrone method gives the best results among the other tried spectrophotometric methods

Improved spectrophotometric determination of disaccharides, hexoses and pentoses using aniline and its alkyl derivatives

Aniline and its alkyl derivatives in acetic acid media react with hexoses and pentoses to form colored products with characteristic absorption spectra. The spectral for individual hexoses, pentoses and their dehydration products and tend to vary with a given aniline derivative. The chromogen formed showed absorption at wavelength 530, 470 and 330mm. The method was improved and used to determine quantitatively glucoses, fructose, maltose, sucrose and ribose and the result obtained was found to obey Beer's law for a concentration range of [0.4-30mg mL-1] for glucose [0.2-2.2 mg.mL-1] for invert sugar [sucrose], [1-3,4mg.mL-1] for maltose under the specific condition that chosen for determination of each sugar