ABSTRACT
Chamber immunofluorescent technique [CIT] was compared with the serum neutralization [SNT] and enzyme-linked immunosorbent assay [ELISA] for the detection of rinderpest virus antibodies in bovine sera. ELISA was found to be the most sensitive test, while CIT compared with SNT was more specific than ELISA. Also, results of CIT were obtained in less than 2 hours. Moreover, titers detected by CIT were comparable to those obtained by SNT. In conclusion, CIT was found to be a reliable, rapid, specific and sensitive technique for the detection of rinderpest antibodies in bovine sera
Subject(s)
Fluorescent Antibody Technique/methodsABSTRACT
Twenty-four sheep of local breeds were used in order to determine the proper FMD virus concentration in vaccinal dose. Four batches of vaccines with different concentration of virus particles [10, 20, 30, and 40 million infective particles per each dose] were prepared and used for sheep vaccination. All sheep challenged by FMD virus [10 4 ID50] gave protection at 21-day post vaccination. Meanwhile, the study of immune response by means of serum neutralizing and complement fixing antibody titers revealed that high titer within protective level [0.9 - 1.2 TCID 50/ml] when sheep vaccinated by dose of 10 million virus particles per dose protected sheep for 5 weeks while when vaccinated by 20 million/dose protection level continues up to 12 weeks, in the same time, sheep vaccinated with dose of 30 and 40 million virus particles per dose gave immunity in protection level up to 24 weeks. The most proper antigenic dose was 30 million virus particles per dose