Modulation of gonadotrophin release from pretreated male camel pituitary cell culture with inhibin and both calcium agonist and antagonist

The present study was conducted to identify the modification carried out by both calcium agonist [Cacl2] and antagonist [VHcI] and inhibin like material [OTLP4] on the GnRH stimulated release of FSH and LH from male camel pituitary cell culture collected during breeding and non breeding seasons. The data obtained showed that 100 and 200 ul/ ml of GnRH was able to release both FSH and LH from pituitaries collected during the year. OTLP4 as an inhibin-like preparation was able to block FSH release from the pituitary especially during non breeding season. Calcium chloride pretreatment has a pronounced effect on LH release without any effect on FSH. The higher dose of calcium chloride unable to release LH from pituitaries collected during breeding season and it may be due to the calcium desensitizing effect. VHcl has a potent effect in blocking LH release to the media by all doses used. The blocking effect on FSH was obtained only when VHcI was used in a higher dose. The use of VHcl in the field for treatment of cardiovascular diseases and its effect on male reproduction need further in vivo investigation

effect of different neurotransmitters on-some hormonal profile of immature female rats

The first objective of the present investigation was to evaluate the relative contributions of the ovarian steroid in the feed back limb of the ovarian pituitary axis. The second was to test the ability of morphine or L. dopa or both in modulating PRL, PROG, FSH and LH levels noticed in female rats previously treated with estrogen during prepubertal period. Forty immature female rats were divided into five equal groups and exposed to different treatments: 1] Rats were injected i.m. with 0.2 ml olive oil and served as a control group; 2] Rats were injected with 5 mug estrogen in 0.2 ml olive oil/day; 3] Rats were injected i.m. with 5 mug estrogen in 0.2 ml olive oil/day/rat + morphine sulfate at a dose pf 10 mg/kg b. wt.; 4] Rats were injected i.m. with both 5 mug estrogen in 0.2 ml olive oil/day/rat + L. dopa at a dose of 30 mg/rat/day; 5] Rats were injected i.m. with 5 mug estrogen in 0.2 ml olive oil/day/rat + morphine sulfate at a dose of 10 mg/kg b. wt. + L. dopa at a dose of 30 mg/rat/day. Animals were inspected daily for vaginal opening and estrous manifestation. Blood samples were collected at the end of experimental period and estimated by radioimmunoassay kits for PRL, LH, FSH and PROG. The data obtained revealed that sexual precocity elicited either with estrogen or estrogen +/- L. dopa treatment of the neurotransmitter in hypothalamic sensitivity to estrogen in induction of puberty may be considered

effect of sex steroids on parathyroid hormone [PTH] and some serum electrolytes in Hubbard chickens

In the present study, twenty four, 8 weeks old Hubbard male chickens were used, to clarify the effect of estradiol benzoate and testosterone propionate on serum Ca, P, Mg, Na and K and also tissue and blood parathhormone [PTH]. The results obtained revealed that, estradiol benzoate significantly decreased serum Ca, Mg, K and PTH in serum and the studied tissues. Testosterone propionate injection significantly increase serum and glandular PTH with a significant decrease in serum P and Mg. The differences between the data obtained as a result of sex steroid injection were conducted to conclusion that steroids play a role in the enbryological characters which are related to the binding protein of calcium [CaBP]

Thyrotrophic [TSH], thyroxine [T4], triiodothyromine [T3] and some other blood and egg parameters as affected by administration of antiadrenergic agent [reserpine] into Fayoumi laying hens

The present study was undertaken to clarify the effect of reserpine on thyroid gl and activity and persistency of egg production besides some other blood and egg parameters at later stages of egg production. Therefore, 50 Fayoumi layers 63 weeks of age were used. The birds were allocated into five equal groups. One kept as control, while the others received different gradient doses of reserpine for nine successive weeks. The results showed that reserpine led to a decrease in serum T4 in all treated groups, while T3 was decreased in two treated groups. TSH was increased in two groups offered reserpine at rate of 3 mg and 6 mg/15 kg feed. Serum cholesterol was increased in three of the treated groups, while total lipids concentration was increased in two of the reserpine treated groups. Reserpine in small doses daily or every other day led to an increase in egg production with a consequent increase in persistency of productivity than that of the controls but it led to a decrease in shell thickness. Thus, it is concluded that reserpine in small doses may modulate the other factors involved in egg production to increase productivity even in slight decrease of thyroid hormones. It is also recommended the use of reserpine in small dose 1.5 mg/15 kg feed day after day to increase egg production for table purposes at the alter stages of flock productivity