The efficacy of immediate implantation of macroporous poly(N-[2-hydroxypropyl]-methacrylamide) hydrogel after laceration spinal cord injury in young rats / Eficacia de la implantación inmediata de hidrogel macroporoso de poli (N- [2-hidroxipropil] - metacrilamida) después de una lesión de la médula espinal en ratas jóvenes

SUMMARY: Spinal cord regeneration after mechanical injury is one of the most difficult biomedical problems. This article evaluates the effect of poly(N-[2-hydroxypropyl]-methacrylamide) hydrogel (PHPMA-hydrogel) on spinal cord regeneration in young rats after lateral spinal cord hemi-excision (laceration) at the level of segments T12-T13 (TrGel group). The locomotor function score (FS) and the paretic hindlimb spasticity score (SS) were assessed according to Basso-Beattie-Bresnahan (BBB) and Ashworth scales, respectively, and compared to a group of animals with no matrix implanted (Tr group). Regeneration of nerve fibers at the level of injury was evaluated at ~5 months after spinal cord injury (SCI). One week after the SCI induction, the FS on the BBB scale was 0.9±0.5 points in the Tr group and 3.6±1.2 points in the TrGel group. In the Tr group, the FS in 5 months was significantly lower than in 2 weeks after SCI, while no significant changes in FS were detected in the TrGel group over the entire observation period. The final FS was 0.8±0.3 points in the Tr group and 4.5±1.8 points in the TrGel group. No significant changes in SS have been observed in the TrGel group throughout the experiment, while the Tr group showed significant increases in SS at 2nd week, 6th week, 3th month and 5th month. The SS in 5 months was 3.6±0.3 points on the Ashworth scale in the Tr group and 1.8±0.7 points in the TrGel group. Throughout the observation period, significant differences in FS between groups were observed only in 5 weeks after SCI, whereas significant differences in SS were observed in 2, 3 and 6-8 weeks post-injury. Glial fibrous tissue containing newly formed nerve fibers, isolated or grouped in small clusters, that originated from the surrounding spinal cord matter have been found between the implanted hydrogel fragments. In conclusion, PHPMA-hydrogel improves recovery of the hindlimb locomotor function and promotes regenerative growth of nerve fibers. Further research is needed to clarify the mechanism of this PHPMA-hydrogel effect.

RESUMEN: La regeneración de la médula espinal después de una lesión mecánica es uno de los problemas biomédicos más difíciles. Este artículo evalúa el efecto del hidrogel de poli (N- [2-hidroxipropil] -metacrilamida) (PHPMA-hidrogel) sobre la regeneración de la médula espinal en ratas jóvenes después de la hemiescisión lateral de la médula espinal (lesión) a nivel de los segmentos T12 - T13 (Grupo TrGel). La puntuación de la función locomotora (FS) y la puntuación de espasticidad parética de las patas traseras (SS) se evaluaron de acuerdo con las escalas de Basso- Beattie-Bresnahan (BBB) y Ashworth, respectivamente, y se compararon con un grupo de animales sin matriz implantada (grupo Tr). Se evaluó la regeneración de las fibras nerviosas al nivel de la lesión ~ 5 meses después de la lesión de la médula espinal (LME). Una semana después de la inducción de SCI, el FS en la escala BBB fue 0,9 ± 0,5 puntos en el grupo Tr y 3,6 ± 1,2 puntos en el grupo TrGel. En el grupo Tr, el FS en 5 meses fue significativamente menor que en 2 semanas después de SCI, mientras que no se detectaron cambios significativos en FS en el grupo TrGel durante el período de observación. El FS final fue de 0,8 ± 0,3 puntos en el grupo Tr y de 4,5 ± 1,8 puntos en el grupo TrGel. No se han obser- vado cambios significativos en SS en el grupo TrGel durante el experimento, mientras que el grupo Tr mostró aumentos significativos en SS en la 2ª semana, 6ª semana, 3º mes y 5º mes. La SS en 5 meses fue de 3,6 ± 0,3 puntos en la escala de Ashworth en el grupo Tr y de 1,8 ± 0,7 puntos en el grupo TrGel. A lo largo del período de observación, se observaron diferencias significativas en FS entre los grupos solo en 5 semanas después de la LME, mientras que se observaron diferencias significativas en SS en 2, 3 y 6-8 semanas después de la lesión. Entre los fragmentos de hidrogel implantados se observó tejido fibroso glial que contenía fibras nerviosas recién formadas, aisladas o agrupadas en pequeños grupos, que se originaban a partir de la materia de la médula espinal circundante. En conclusión, PHPMA-hydrogel mejora la recuperación de la función locomotora de las patas traseras y promueve el crecimiento regenerativo de las fibras nerviosas. Se requieren más estudios para aclarar el mecanismo del efecto de hidrogel PHPMA.

Malaria: a general minireview with reference to Egypt

The majority of world's population-live in areas at risk of malaria transmission. Malaria is a serious Anopheles-borne disease that causes symptoms like the flu, as a high fever, chills, and muscle pain also, anemia, bloody stools, coma, convulsion, fever, headache, jaundice, nausea, sweating and vomiting. Symptoms tend to come and go in cycles. Apart from Anopheles vector, malaria could be transmitted nosocomial, blood transfusion or needle-stick injury Some types of malaria may cause more serious damage problems to heart, lungs, kidneys, or brain. These types can be deadly. The primary factors contributing to the resurgence of malaria are the appearance of drug-resistant strains of the parasite, the spread of insecticideresistant strains of the mosquito and the lack of licensed malaria vaccines of proven efficacy. In rare cases, people can get malaria if they come into contact with infected blood as in blood transfusion or needle-stick injury also nosocomial and congenital malaria was reported. This is a mini-review of malaria with information on the lethal to humans, Plasmodium falciparum, together with other recent developments in the field

Patient Dose Assessment for Common Digital Diagnostic Radiology Examination in Hamad Medical Corporation Hospitals in the State of Qatar.

The aim of this study was to estimate radiation doses to patients undergoing standard radiographic examinations using Computed Radiography (CR) and Direct Digital Radiography (DDR) in two hospitals within Hamad Medical Corporation (HMC) in Qatar, and compare the results with regional and international Diagnostic Reference Levels (DRLs). Data on 3391 patients were recorded from different X-ray rooms in HMC hospitals. Entrance Skin Dose (ESD) was measured for 1046 patients for the most five common X-ray examinations (a total of 7 projections) namely: Skull, Chest, Abdomen, Lumbar Spine and Pelvis. Exposure factors such as kV, mAs and Focal to Skin Distance (FSD) were recorded for each patient. Tube Output was measured for a range of selected kV values. ESD for each individual patient was calculated using the tube output and the technical exposure factors for each examination. The ESD values were compared with some international DRLs for all types of examinations. HMC patient demographic data evaluated from this study were: average age of 39 years, average weight of 60-80 kg and mean height of 165 cm. The most procedure performed during the time of this study was chest PA (52%), and the least procedure performed was skull AP/LAT (1%) examination. The mean ESD values found to be generally lower than the published values. With exception of abdomen examinations at Hamad General Hospital (HGH), mean ESD values were found to be within the established IAEA (DRL). The mean ESD values at HGH were found to be much higher than that at Al Khor Hospital (AKH) for abdomen, Lumbar spine AP, Lumbar Spine LAT and pelvis, but generally lower than the published values.

Evaluation of Mean Glandular Dose from Digital Mammography Exams at Qatar and Compared with International Guidelines Levels.

The primary objective of this study was to measure the mean glandular dose (MGD) from craniocaudal (CC) and mediolateral oblique (MLO) views from mammography patients in Hamad Medical Corporation (HMC) in the state of Qatar and to compare them with the international guidelines levels as well as to establish Dose Reference Level (DRL) for the country by applying the quality control (QC) protocol for the Digital mammography units and to reduce the patient dose and improve the image quality. All patients data was taken from two Selenia digital mammography units for 18 months period. Quality control was implemented for the two mammography machines and corrective actions have been done for the image quality evaluation with rejected film analysis. The total number of collected patient data was 4085 mammography exams which considered as around 93% of the overall mammography procedures done in Qatar during that period. Based on the IAEA selection criteria of breast thickness between 2-7 cm and kV machine value from 26 to 33 kV, only 3280 mammography procedures satisfies the above criteria and are analysed accordingly, National Centre for Cancer Care & Research (NCCCR) 949 and Hamad General Hospital (HGH) 2331 exposures. The present study revealed that there were significant differences between the MGD values for the CC and MLO views (p <0.006). Referring to the limiting dose values in the European guidelines, the results from the two mammography units showed that 94.5% and 99.7% of the mean glandular doses are acceptable from NCCCR and HGH respectively. Due to compression devise error in the NCCCR mammography machine, the MGD for some patients became more than the acceptable values especially at small breast thickness values (0-3 cm) which it seems 0% in the acceptable range.

Human babesiosis: a general review with special reference to Egypt

Babesiosis is a tick-borne malaria-like illness caused by species of the intra-erythrocytic protozoan Babesia. Humans are opportunistic hosts for Babesia when bitten by nymph or adult ticks. Currently, Babesia infection is transmitted by various tick vectors in Europe, Asia, Africa and the northwestern and northeastern United States. Human babesiosis was first described in 1957 but is now known to have worldwide distribution. The increase in reported cases is likely due to increases in actual incidence as well as increased awareness of the disease. Despite the diagnostic and preventive advances resulting from extensive research and a greater understanding of the disease, babesiosis continues to have significant medical impact as a confounding variable in the diagnosis and treatment of Lyme disease and as a potential threat to the blood supply, especially in the United States. Diagnostic advances, like the development of PCR assays, have resulted in increased sensitivity for detection as well as the discovery and characterization of new babesial species. Further studies using the molecular tools now available and those to be developed will lead to a better understanding of the natural history of these organisms, including the transmission cycle and the potential role of Babesia parasites themselves as immunomodulator. Human babesiosis is usually an asymptomatic infection in healthy individuals. Several patients become symptomatic, and, within these subpopulations, significant morbidity and mortality occur, especially in elderly, immunocompromised, or asplenic patients. It is difficult to diagnose. Although the index of suspicion should be high in rural Babesia endemic areas, patients with babesiosis have few, if any, localizing signs to suggest the disease. Diagnosis confirmation depends on the parasitemia degree, the expertise and well trained laboratory personnel. Most patients infected by B microti who are otherwise healthy appear to have a mild illness and typically recover without specific chemotherapy, however, treatment is recommended for all diagnosed cases to prevent sequelae and potential transmission through blood donation. In addition, patients should be advised to take precautions against tick exposure and to refrain from donating blood until completely cured of babesiosis

Selected infectious disease disasters for nursing staff training at Egyptian eastern border

Infectious disease disasters are events that involve a biological agent, disease and that result in mass casualties, such as a bioterrorism attack, an emerging outbreak of infectious disease; all disasters pose a risk of infection transmission. But, infectious disease disasters pose the great-risk to illness or death from an infectious disease. This study raised the awareness and improved knowledge by educational program for Military Nursing Staff on selected infectious disease disasters acquired at Egyptian Eastern Border. The selected arthropodborne diseases were Anthrax, Tick borne relapsing, Louse borne replasing fever and liver fluke; Clonorchis sinensis. An interventional study was used, for 125 staff nurse who accepted to participate. The tools dealt with four questionnaires: [1] Some socio-demographic characteristics data [2] Educational needs assessment a structured questionnaire. [3] Knowledge test [pre/ post-test] and [4] Participants' reactions questionnaire. The results showed that educational intervention significantly improvements the nursing staff knowledge, which were achieved at the immediate post intervention phase, and retained via three months post-test phase. In the service training programs about infectious disease disasters at Egyptian Eastern Border must be established and continued on regular basis. This would improve their knowledge about the epidemiology of these infectious disease disasters

Study of the hepatoprotective effect of Juniperus phoenicea constituents

Different fraction obtained from the aerial parts of Juniperus phoenicea showed significant activity as hepatoprotective when investigated against carbon tetrachloride induced liver injury. The hepatoprotective activity was evaluated through the quantification of biochemical parameters and confirmed using histopathology study. Phytochemical investigation of the petroleum ether, chloroform and methanol fractions utilizing different chromatographic techniques resulted in the isolation of five known diterpenoids namely: 13-epicupressic acid [1], imbricatolic acid [2], 7 alpha -hydroxysandaracopimaric acid [3], 3 beta -hydroxysandaracopimaric acid [4], isopimaric acid [5], four flavonoid derivatives: cupressuflavone [6], hinokiflavone [7], hypolaetin-7-O- beta -xylopyranoside [9], [-] catechin [10], inaddition to sucrose [8]. Both physical and spectral data were used for structure determination and all isolates were evaluated for their hepatoprotective activity. Compounds 2 and 6 were effective, however; 7 was the most active. Hepatoprotective activity of 7 is comparable with the standard drug silymarin in reducing the elevated liver enzymes and restoring normal appearance of hepatocytes. Hepatoprotective effect of combination of 6, 7 and silymarin with the diterpene sugiol was also explored

freshwater ampullariid snail canistes carinatus as a natural intermediate host of angiostrongylus cantonensis in Egypt

The rate and density of infection with Angiostrongylus cantonensis in Lanistes carinatus were studied in Assuit, Benisowif and Behyra Governorates representing Upper, Middle and Lower Egypt respectively. Snail samples collected in summer and winter were found variably infected with third - stage larvae of this parasite indicating spread of the parasite allover the country. Prolonged desiccation of snails up to four months or starvation up to 20 days proved to have little or no considerable effect on the survival of the borne larvae under laboratory conditions. The distribution pattern of the third stage larvae of the parasite within the body of naturally infected snails is also given