Some histological, histochemical and ultrastructural studies on the effect of tobramycin on the convoluted tubules of the kidney of albino rat

Tobramycin is the most active member of all the aminoglycoside antibiotic group acting against gram- negative bacteria. The aim of present study was to detect the effect of tobramycin on tile convoluted tubules in two different dosage regimen. Sixty adult male albino rats were divided into three groups. Group I as control. Group II: included rats which were injected intraperitoneally with daily dose of 4 mg/kg tobramycin in three equally divided doses. Group III: injected with the same previous dose once daily. They were sacrificed after 3,10,14 days of injection and tissue samples from the cortex of each kidney were taken and processed for both light and electron microscopic examination. In group II, by light microscope swelling of the cells lining the convoluted tubules and vacuolation of their cytoplasm as well as brown granules and hemorrhage were observed. The regenerating cells appeared with basophilic cytoplasm. Progressive decrease in succinic dehydrogenase and increase in acid phosphatase enzymatic activities were observed. Ultrastructurally, there were affection of mitochondria and partial or complete loss of microvilli in some tubules were observed. The cytoplasm of some tubules revealed numerous myeloid bodies with various degrees of nuclear degeneration. Dilated basal infoldings and thickened basement membrane was also observed. The previous changes were less evident on administration of the drug in Group III. In conclusion, patients with a previous kidney disease should better avoid the use of this drug whenever possible but if its use is necessary, once daily dosage may be advised for short period

Histological and histochemical study of the entero-endocrine cells in the gastrointestinal tract of adult male albino rats

Ten adult male albino rats were sacrificed and specimens were taken from different parts of the gastrointestinal tract. Each specimen was divided into three parts; the first was fixed in 10 percent formalin, the second in Bouin and the third part in Regaud's fluid. Chromaffin reaction and its modification, Giemsa stain, Argyrophil reaction and Azocoupling reaction were used for identification of entero endocrine cells in the gastrointestinal epithelium. The argyrophil cells appear more numerous than argentaffing cells in the bases of the glands the fundus and pylorus and in the crypts of small and large intestine Their shape varied from triangular to rounded or columnar with has granules and apical processes. The duodenum is the richest site entero-endocrine cells followed by the jejunum, pylorus, proximal and distal colon, fundus of stomach and finally the appendix

Histological study of the effect of hypervitaminous D on some organs of albino rat

Adult male albino rats were injected with variable doses of vitamin [D] for different periods sections from their kidneys and aortae were then stained with HX and E and Van Gieson's stain. Marked damage of the kidney was observed in the form of atrophy of the capillary tuft, thickening of Bowman's capsules, irregular dilatation of the renal tubules with areas of haemorrhage and cellular infiltration of the intestinal tissue with deposition of acidophilic and basophilic materials. Deformity of the walls of the aorta was noticed with destruction of its elastic lamellae and deposition of basophilic materials. High toxic doses of vitamin [D] led to distortion of the wall with aneurismal formation and appearance of hyaline cartilage like tissues inside its wall